DOMIICANA IMPORTANT INFORMATION ON THE SWINE FLU "swine flu"
the flu?
swine influenza (flu) is a respiratory disease of pigs caused by influenza virus type A, which causes outbreaks of influenza among animals. The swine influenza virus in pigs seriously ill but mortality rates are low. These viruses can spread among pigs throughout the year, but most outbreaks occur in the final months of autumn and winter, as well as outbreaks in humans. The virus of classical swine flu (H1N1 influenza virus type A) was first isolated from a pig in 1930.
How many swine influenza virus are there?
Like all viruses of influenza, swine influenza viruses are constantly changing. Pigs can be infected by avian influenza viruses and human, as well as for the swine influenza virus. When influenza viruses from different species infect pigs, the virus can be grouped (ie swap genes) and new viruses may arise from the mixture of swine influenza virus with human influenza, or bird. Through the years, there have been various changes in swine influenza virus. Currently, there are four main subtypes of influenza virus isolated in pigs: H1N1, H1N2, H3N2 and H3N1. However, most influenza viruses recently isolated from pigs have been the H1N1 virus.
human swine influenza
"Humans can get swine flu?
The swine influenza viruses usually do not infect humans. However, there have been sporadic cases of swine influenza infections in humans. Usually, these cases occur in people who have direct exposure to pigs (ie, children who go to fairs or pigs in the swine industry workers). In addition, there have been some documented cases of people who have caught the virus of swine influenza to others. For example, in 1988, a suspected outbreak of swine flu infection in pigs in Wisconsin caused multiple human infections and, although there was an outbreak in the community, is antibody evidence of virus transmission from patient to patient care staff who had close contact with him.
How often swine flu infection in humans?
In the past, the CDC received reports of approximately one case of infection with swine influenza virus in humans every one to two years in the United States, however, from December 2005 to February 2009 have reported 12 cases of swine influenza infections in humans.
What are the symptoms of swine flu in humans?
The symptoms of swine flu in people are similar to those of seasonal influenza in humans and these include fever, lethargy, poor appetite, and cough. Some people with swine influenza have also been reported runny nose, sore throat, nausea, vomiting and diarrhea.
Can people swine flu from eating pork?
No. swine influenza virus is not transmitted by food. You may not swine flu from eating pork or pork products. There are no risks if you eat pork and its derivatives have been handled and cooked properly. If you cook pork to an internal temperature of 71 ° C (160 ° F) eliminating the swine influenza virus, as well as other bacteria and viruses.
How is it spread the flu?
influenza viruses can be transmitted directly from pigs to people and people to pigs. Infections in humans by the influenza virus from pigs are more likely to occur in people who are in close contact with infected pigs, including those working on pig farms and those involved in pig houses fairs, livestock exhibits. The swine influenza transmission from person to person can also occur. It is believed that this transfer is equal to that of seasonal influenza in people, ie mainly from person to person when infected by the virus of influenza cough or sneeze. People can become infected by touching something with influenza virus and then touching their mouth or nose.
What we know about human transmission of swine influenza from person to person?
In September 1988, a pregnant woman healthy 32-year-old was hospitalized for pneumonia and died eight days later. The swine influenza virus H1N1 was detected. Four days before becoming ill, the patient had visited an exhibition of pigs at a county fair where widespread influenza-like illness in pigs.
In follow-up studies, 76% of exhibitors from pigs which were tested showed antibody evidence of swine flu infection, although this group did not detect major diseases. Additional studies suggest that one to three employees of the medical staff who had contact with the patient developed mild flu-like illness and antibodies to swine influenza infection.
How are diagnosed with swine flu infections in humans?
To diagnose an infection with swine influenza A, usually must collect a sample of respiratory secretions between the first 4 to 5 days of illness (when an infected person is more likely to spread the virus). However, some people, especially children, can spread the virus for 10 days or more. For the identification of swine influenza virus type A is necessary to send the sample to the CDC for laboratory testing.
What medications are available to treat influenza infections in swine?
There are four different antiviral drugs that are approved in the United States for the treatment of influenza: amantadine, rimantadine, oseltamivir and zanamivir. Although the majority of swine influenza virus have been sensitive to the four types of drugs, the most recent virus swine influenza viruses isolated from humans are resistant to amantadine and rimantadine. Currently, the CDC recommends the use of oseltamivir or zanamivir for the prevention and treatment of infection with swine influenza virus. You can find more information on recommendations for treatment on site www.cdc.gov / flu / swine / recommendations.htm.
What other cases of swine flu outbreaks there?
Probably the best known case is an outbreak of swine flu among soldiers at Fort Dix, New Jersey, in 1976. The virus caused disease, x-ray evidence at least 4 soldiers and 1 death, all these patients had previously been healthy. The virus was transmitted to close contacts in an environment of basic training, and no transmission outside the basic training group. It is believed that the virus remained there a month and disappeared. The source of the virus, the exact date of his admission to Fort Dix, and factors limiting its spread and duration. The Fort Dix outbreak may have been caused by introduction of a virus from one animal to human populations under stress in close contact in crowded facilities and during the winter. The swine influenza virus type A contained a Fort Dix soldier was baptized A / New Jersey/76 (Hsw1N1).
Does swine influenza virus H1N1 is equal to the H1N1 influenza virus in humans?
No. swine influenza virus H1N1 viruses are antigenically very different from the H1N1 virus in humans, therefore, vaccines for seasonal influenza for persons not provide protection against swine influenza virus H1N1. Swine influenza in pigs
How swine flu spread among pigs?
is believed that the swine influenza virus is spread mainly through close contact between pigs and possibly contaminated objects moving between infected and uninfected pigs. Pig herds with continuous swine influenza infections and herds that are vaccinated against this disease may have sporadic disease, may have no symptoms or only mild symptoms of infection.
What are the signs of swine influenza in pigs?
signs of swine flu may include sudden onset of fever, depression, cough (groan), runny nose and eyes, sneezing, difficulty breathing, eye redness or swelling and loss of interest in food.
How often is the swine influenza in pigs?
viruses H1N1 and H3N2 swine influenza is endemic among pig populations in the United States and is one that the industry deals with routinely. Outbreaks among pigs normally occur usually in cold weather months (late fall and winter) and sometimes with the introduction of new pigs into susceptible herds. Studies have shown that the H1N1 swine influenza is common in pig populations worldwide and that 25 percent of the animals show signs of antibodies against infection. Studies in the United States have shown that 30 percent of the population of pigs tested have presented evidence of antibodies for H1N1 infection. To be more precise, has proven the presence of antibodies to H1N1 infection in 51 percent of pigs in north central United States. Infections in humans by the H1N1 virus of swine influenza are rare. At present, there is no way to differentiate in pigs produced antibodies in reaction to the vaccination of antibodies generated against the H1N1 swine influenza infections.
Although swine influenza virus H1N1 have been found in pig populations since at least 1930, swine influenza virus H3N2 did not begin circulating among pigs in the United States until 1998. The H3N2 virus first entered the pig populations by humans. The current influenza virus swine H3N2 viruses are closely related to the H3N2 virus in humans.
Is there a vaccine for swine flu? There
vaccines given to pigs to prevent swine influenza. However, there is no vaccine to protect people against influenza virus. It is possible that seasonal influenza vaccine provides partial protection against H3N2 virus but not against the H1N1 influenza virus.
(Source: Center for Infectious Disease Coordinator CCID)
(National Center for Immunization and Respiratory Diseases (NCIRD)
(Preparation and Planning Pandemic Flu) (Flu)
Tuesday, April 28, 2009
Saturday, April 18, 2009
I Like Touching My Breasts
Mycorrhizae FOOD SAFETY
Introduction
mycorrhizae are called the organs formed by the root of a plant and the mycelium of a fungus. Its function is to absorb, so that spread along the ground to provide water and nutrients and protecting the roots of some diseases.
In return, the fungus gets the sugar it needs, from plant photosynthesis. So, thanks to the performance of mycorrhizae, growth is enhanced and improved endurance. Most plants perform this symbiosis with fungi, for which it is necessary that the environmental conditions are favorable to both.
Importance of Mycorrhizae for Ecosystem
Mycorrhizae play a key role in the terrestrial ecosystem, playing a number of functions essential to the health of many plants and crops. And is that the role of the fungus is biotrophic colonize the bark of a particular result, without causing any harm, but rather integrates becoming part of it. In turn, the fungus also colonizes the soil around the root using the external mycelium, so that helps the host to acquire mineral nutrients and water.
For its part, the plant provides the fungus with carbon compounds that come from photosynthesis. For this reason, develop mycorrhizae a key role in the development and maintenance of many ecosystems, so that can be found in all soils and all land climates. Because of the role played mycorrhizae as protectors of crops can reduce fertilizer and herbal medicine in those plants that possess them.
Mycorrhizal Fungi mutualistic association that occurs between the fungus and root is used by the majority of vascular plants and fungi-like basidiomycetes, ascomycetes and zygomycetes. Much of edible mushrooms in the forest of pine, as the nízcalo, the brats, the death cap mushroom, the bold, pomfret or cat's tongue, are fruiting bodies of these fungi. Arbuscular Mycorrhizae
most common mycorrhizal fungi are arbuscular, which provide major economic benefits due to its beneficial effect on growth and stress tolerance of a large crop. Here are some micro earthquakes that are characterized by their inability to reproduce except by the colonization of plant roots, which is called the host.
fungi that lead to this type of mycorrhizae are microscopic Zygomycetes Glomales order. Forming arbuscular mycorrhizae are most herbaceous and some woody legumes, cereals, fruit, the vast majority of horticultural crops and many shrubs, dwarf shrubs and herbs of forest ecosystems. Degraded Soil Benefit
Another major role of mycorrhizae is assisting in the establishment and protection of those plants that are less productive soils, as affected by desertification, pollution by heavy metals or salinity. Thus, it provides many benefits to crops and yields healthy food. Thus, arbuscular mycorrhizae can stop soil erosion and desertification, especially in Mediterranean ecosystems.
On the other hand, in soils affected by the negative effects of heavy metals have shown that mycorrhizal plants have greater resistance, with the ability to get to immobilize the metals in the roots, preventing them from passing to the shoot of the plant. Finally, with regard to salinity should be noted that currently are conducting studies that indicate that these fungi are more appropriate for this factor.
Mycorrhizae: "Fungus-root",
Mycorrhizae are organs formed by the root of a plant and the mycelium of a fungus. Function as an absorption system which extends along the ground and is able to provide water and nutrients (nitrogen and phosphorus in particular) to the plant roots and protect against some diseases. The fungus in turn receive plant sugars from photosynthesis. There are thousands of species of fungi that form mycorrhizal symbiosis with trees. Most families of higher plants mycorrhizal Earth.
What are mycorrhizae? The term mycorrhiza
globally describes a series of structures formed by the partnerships established between various kinds of soil fungi and the roots of most vascular plants (Frank, 1885), and has even been described on non-vascular plants .
As a widespread phenomenon, the term "mycorrhiza" has become at the user level in the name designating the fungi involved in their formation, although this name is not very correct, these same routines have been coined colloquial terms such as "mycorrhizal 'fungi make contact mycorrhizal plants and 'mycorrhization' to indicate the establishment of symbiosis.
How They Work?
type in these mutualistic symbiosis, the fungus supplies the plant inorganic compounds (minerals) it needs for nutrition (mycotrophy) and the plant provides the fungus heterotrophic organic compounds (photosynthates).
The establishment of these associations involves creating strong interdependencies, so much so that the fungus becomes a part of the root system, so perfectly integrated into the same looks very difficult or even impossible to develop without the assistance of its host plant, and it may have Range dependence of the fungus, ranging from absolute to relative to a greater or lesser degree (Barea et al., 1993).
How many types of mycorrhizae exist?
generally allowed five types of mycorrhizae (Harley and Smith, 1983), namely:
Ectomycorrhizae: The fungi that form, Basidiomycetes and Ascomycetes, develop a thick layer of mycelium on the cortical area feeder roots of the plant. Occur mainly on forest and woody species.
Endomycorrhizae: The fungi that produce them are characterized by intracellularly colonize the root cortex. Within this group there are three distinct types:
Orquideomicorrizas (associated with Orquidiáceas).
Ericomicorrizas (linked to the family Ericaceae, with many structural similarities with
ectendomicorrizas.
arbuscular mycorrhizae: Characterized by forming intracellular arbuscules and certainly the most widespread and economic and ecological importance.
Ectendomicorrizas:
fungi that produce dual colonize the roots: externally forming a cortical mantle and internally penetrating intracellularly in the cortex.
On what plants are established?
mycorrhizal associations occurring on almost all vascular plants with a few exceptions such as the families Cruciferae, Chenopodiaceae, Cyperaceae, Caryophyllaceae and Juncaceae (Azcón Aguilar and Barea, 1997) and also set in Bryophytes and Pteridophytes, although there is little information on these symbiosis with vascular plants (Moser and Haselwandter, 1983).
Among the vascular plants colonized by 'mycorrhiza' are all woody species of forestry interest (Fagaceae, Betulaceae, Pinaceae, etc.), all species of horticultural interest (Solanaceae, grass, etc..) and many families of ornamental importance (Orquidiáceas, Rosaceae ...). This gives a clear idea of \u200b\u200bthe ecological and economic importance of mycorrhizae.
What are the Major Categories of mycorrhizal fungi?
• In ectomycorrhizae: Suillus, Cortinarius, Rhizopogon, Cenococcuym, Thelefora, Pisolithus. • In orquideomicorrizas
: Armillariella, Gymnopilus, Marasmius, Fomes, Xerotus, Ceratobasidium, Corticium, Sebacina, Tulasnella. • In ericomicorrizas
: Pezizella.
• In arbuscular mycorrhizae: Acaulospora, Entrophospora, Gigaspora, Glomus, and Scutellospora Sclerocystis. • In ectendomicorrizas
: Endogone. How to Produce
Colonization?
At first there is a mutual identification plant fungus in the rhizosphere, in regions close to the feeder roots, this recognition seems mediated by substances exuded by the root cause of the mycelium growth and a positive biotropism it towards the root. Then there is the intercellular contact to form a structure called appressorium Thirdly, morphological and structural changes in tissues colonized by the fungus, and the organization of the cell wall of the symbiont fungi. Is subsequently physiological integration of both symbionts, and finally there is an alteration of enzymatic activities that are coordinated between the symbionts to integrate metabolic processes (). Is
Specificity in the Formation of Mycorrhizae? While
mycorrhizal associations are generally considered non-specific, meaning that any symbiotic fungus can colonize any plant receptive, however there are "preferences" or better compatibility between certain pairs affinity fungus / plant. In contrast there are also cases such as Eucalyptus, Malus, Arbutus (Meyer, 1973) that the total associative specificity makes these and other species are colonized at the same time as different as ectomycorrhiza formation and endomycorrhizas.
What are the Effects of mycorrhizal associations?
Mycorrhizae act at several levels, causing morphological and anatomical changes in the host plants such as changes in root stem ratio in root tissue structure, the number of chloroplasts, increased lignification, disruption of hormonal balances. .., effects that are not only explicable as a mere improvement of plant nutrition due to increased efficiency of nutrient uptake by the root through to the formation of mycorrhizae, but responds to metabolic changes deeper and more complex due to physiological integration of symbionts.
Another most interesting effects of mycorrhizae is their role in the ecosystem in which they develop, and interact with various microorganisms mycorrhizosphere establishment for profitable cooperation with each other and generally competing with pathogenic type, and even interacting with rhizosphere microfauna (nematodes, aphids, mites, ...), but its apparent protective role is relative (and Haselwandter Moser, 1983).
What are the Practical Applications of Mycorrhizae?
Apart from their scientific, studies of plant physiological type, microbiology, biotechnology applications in commercial production and ornamental horticulture, is to highlight its application to processes of reforestation revegetation (Salamanca et al., 1992) and recovery in arid and degraded soils (Daft et al., 1975).
Another cutting-edge applications is easy to deduce from all this, its application in biological control against pathogens in the rhizosphere.
But the best reason to work with and the satisfaction they offer to their handlers is to get vigorous and healthy plants.
Conclusion
In Nature this symbiosis occurs spontaneously. It is estimated that between 90 and 95% of higher plants have mycorrhizae as usual.
is possible that one fungus forms mycorrhizae with more than one plant at a time, thus establishing a connection between different plants, and this facilitates the existence of parasitic plants (some of whom do not even carry out photosynthesis, such as the genus Monotropa), which extracted all they need mycobiont fungus and other plants with which it also establishes symbiosis. In addition, several fungi (in Sometimes different species) may mycorrhizal same plant at the same time.
www.encuentros.uma.es/encuentros55/micorrizas Bibliographies References.
www.plantasyhogar.com / garden
Carlos López Encina is Scientific Collaborator (CSIC) in the Experimental Station Mayora. Muñoz Araceli Barceló
Researcher
es.wikipedia.org CIFA / wiki / Mycorrhiza
Introduction
mycorrhizae are called the organs formed by the root of a plant and the mycelium of a fungus. Its function is to absorb, so that spread along the ground to provide water and nutrients and protecting the roots of some diseases.
In return, the fungus gets the sugar it needs, from plant photosynthesis. So, thanks to the performance of mycorrhizae, growth is enhanced and improved endurance. Most plants perform this symbiosis with fungi, for which it is necessary that the environmental conditions are favorable to both.
Importance of Mycorrhizae for Ecosystem
Mycorrhizae play a key role in the terrestrial ecosystem, playing a number of functions essential to the health of many plants and crops. And is that the role of the fungus is biotrophic colonize the bark of a particular result, without causing any harm, but rather integrates becoming part of it. In turn, the fungus also colonizes the soil around the root using the external mycelium, so that helps the host to acquire mineral nutrients and water.
For its part, the plant provides the fungus with carbon compounds that come from photosynthesis. For this reason, develop mycorrhizae a key role in the development and maintenance of many ecosystems, so that can be found in all soils and all land climates. Because of the role played mycorrhizae as protectors of crops can reduce fertilizer and herbal medicine in those plants that possess them.
Mycorrhizal Fungi mutualistic association that occurs between the fungus and root is used by the majority of vascular plants and fungi-like basidiomycetes, ascomycetes and zygomycetes. Much of edible mushrooms in the forest of pine, as the nízcalo, the brats, the death cap mushroom, the bold, pomfret or cat's tongue, are fruiting bodies of these fungi. Arbuscular Mycorrhizae
most common mycorrhizal fungi are arbuscular, which provide major economic benefits due to its beneficial effect on growth and stress tolerance of a large crop. Here are some micro earthquakes that are characterized by their inability to reproduce except by the colonization of plant roots, which is called the host.
fungi that lead to this type of mycorrhizae are microscopic Zygomycetes Glomales order. Forming arbuscular mycorrhizae are most herbaceous and some woody legumes, cereals, fruit, the vast majority of horticultural crops and many shrubs, dwarf shrubs and herbs of forest ecosystems. Degraded Soil Benefit
Another major role of mycorrhizae is assisting in the establishment and protection of those plants that are less productive soils, as affected by desertification, pollution by heavy metals or salinity. Thus, it provides many benefits to crops and yields healthy food. Thus, arbuscular mycorrhizae can stop soil erosion and desertification, especially in Mediterranean ecosystems.
On the other hand, in soils affected by the negative effects of heavy metals have shown that mycorrhizal plants have greater resistance, with the ability to get to immobilize the metals in the roots, preventing them from passing to the shoot of the plant. Finally, with regard to salinity should be noted that currently are conducting studies that indicate that these fungi are more appropriate for this factor.
Mycorrhizae: "Fungus-root",
Mycorrhizae are organs formed by the root of a plant and the mycelium of a fungus. Function as an absorption system which extends along the ground and is able to provide water and nutrients (nitrogen and phosphorus in particular) to the plant roots and protect against some diseases. The fungus in turn receive plant sugars from photosynthesis. There are thousands of species of fungi that form mycorrhizal symbiosis with trees. Most families of higher plants mycorrhizal Earth.
What are mycorrhizae? The term mycorrhiza
globally describes a series of structures formed by the partnerships established between various kinds of soil fungi and the roots of most vascular plants (Frank, 1885), and has even been described on non-vascular plants .
As a widespread phenomenon, the term "mycorrhiza" has become at the user level in the name designating the fungi involved in their formation, although this name is not very correct, these same routines have been coined colloquial terms such as "mycorrhizal 'fungi make contact mycorrhizal plants and 'mycorrhization' to indicate the establishment of symbiosis.
How They Work?
type in these mutualistic symbiosis, the fungus supplies the plant inorganic compounds (minerals) it needs for nutrition (mycotrophy) and the plant provides the fungus heterotrophic organic compounds (photosynthates).
The establishment of these associations involves creating strong interdependencies, so much so that the fungus becomes a part of the root system, so perfectly integrated into the same looks very difficult or even impossible to develop without the assistance of its host plant, and it may have Range dependence of the fungus, ranging from absolute to relative to a greater or lesser degree (Barea et al., 1993).
How many types of mycorrhizae exist?
generally allowed five types of mycorrhizae (Harley and Smith, 1983), namely:
Ectomycorrhizae: The fungi that form, Basidiomycetes and Ascomycetes, develop a thick layer of mycelium on the cortical area feeder roots of the plant. Occur mainly on forest and woody species.
Endomycorrhizae: The fungi that produce them are characterized by intracellularly colonize the root cortex. Within this group there are three distinct types:
Orquideomicorrizas (associated with Orquidiáceas).
Ericomicorrizas (linked to the family Ericaceae, with many structural similarities with
ectendomicorrizas.
arbuscular mycorrhizae: Characterized by forming intracellular arbuscules and certainly the most widespread and economic and ecological importance.
Ectendomicorrizas:
fungi that produce dual colonize the roots: externally forming a cortical mantle and internally penetrating intracellularly in the cortex.
On what plants are established?
mycorrhizal associations occurring on almost all vascular plants with a few exceptions such as the families Cruciferae, Chenopodiaceae, Cyperaceae, Caryophyllaceae and Juncaceae (Azcón Aguilar and Barea, 1997) and also set in Bryophytes and Pteridophytes, although there is little information on these symbiosis with vascular plants (Moser and Haselwandter, 1983).
Among the vascular plants colonized by 'mycorrhiza' are all woody species of forestry interest (Fagaceae, Betulaceae, Pinaceae, etc.), all species of horticultural interest (Solanaceae, grass, etc..) and many families of ornamental importance (Orquidiáceas, Rosaceae ...). This gives a clear idea of \u200b\u200bthe ecological and economic importance of mycorrhizae.
What are the Major Categories of mycorrhizal fungi?
• In ectomycorrhizae: Suillus, Cortinarius, Rhizopogon, Cenococcuym, Thelefora, Pisolithus. • In orquideomicorrizas
: Armillariella, Gymnopilus, Marasmius, Fomes, Xerotus, Ceratobasidium, Corticium, Sebacina, Tulasnella. • In ericomicorrizas
: Pezizella.
• In arbuscular mycorrhizae: Acaulospora, Entrophospora, Gigaspora, Glomus, and Scutellospora Sclerocystis. • In ectendomicorrizas
: Endogone. How to Produce
Colonization?
At first there is a mutual identification plant fungus in the rhizosphere, in regions close to the feeder roots, this recognition seems mediated by substances exuded by the root cause of the mycelium growth and a positive biotropism it towards the root. Then there is the intercellular contact to form a structure called appressorium Thirdly, morphological and structural changes in tissues colonized by the fungus, and the organization of the cell wall of the symbiont fungi. Is subsequently physiological integration of both symbionts, and finally there is an alteration of enzymatic activities that are coordinated between the symbionts to integrate metabolic processes (
Specificity in the Formation of Mycorrhizae? While
mycorrhizal associations are generally considered non-specific, meaning that any symbiotic fungus can colonize any plant receptive, however there are "preferences" or better compatibility between certain pairs affinity fungus / plant. In contrast there are also cases such as Eucalyptus, Malus, Arbutus (Meyer, 1973) that the total associative specificity makes these and other species are colonized at the same time as different as ectomycorrhiza formation and endomycorrhizas.
What are the Effects of mycorrhizal associations?
Mycorrhizae act at several levels, causing morphological and anatomical changes in the host plants such as changes in root stem ratio in root tissue structure, the number of chloroplasts, increased lignification, disruption of hormonal balances. .., effects that are not only explicable as a mere improvement of plant nutrition due to increased efficiency of nutrient uptake by the root through to the formation of mycorrhizae, but responds to metabolic changes deeper and more complex due to physiological integration of symbionts.
Another most interesting effects of mycorrhizae is their role in the ecosystem in which they develop, and interact with various microorganisms mycorrhizosphere establishment for profitable cooperation with each other and generally competing with pathogenic type, and even interacting with rhizosphere microfauna (nematodes, aphids, mites, ...), but its apparent protective role is relative (and Haselwandter Moser, 1983).
What are the Practical Applications of Mycorrhizae?
Apart from their scientific, studies of plant physiological type, microbiology, biotechnology applications in commercial production and ornamental horticulture, is to highlight its application to processes of reforestation revegetation (Salamanca et al., 1992) and recovery in arid and degraded soils (Daft et al., 1975).
Another cutting-edge applications is easy to deduce from all this, its application in biological control against pathogens in the rhizosphere.
But the best reason to work with and the satisfaction they offer to their handlers is to get vigorous and healthy plants.
Conclusion
In Nature this symbiosis occurs spontaneously. It is estimated that between 90 and 95% of higher plants have mycorrhizae as usual.
is possible that one fungus forms mycorrhizae with more than one plant at a time, thus establishing a connection between different plants, and this facilitates the existence of parasitic plants (some of whom do not even carry out photosynthesis, such as the genus Monotropa), which extracted all they need mycobiont fungus and other plants with which it also establishes symbiosis. In addition, several fungi (in Sometimes different species) may mycorrhizal same plant at the same time.
www.encuentros.uma.es/encuentros55/micorrizas Bibliographies References.
www.plantasyhogar.com / garden
Carlos López Encina is Scientific Collaborator (CSIC) in the Experimental Station Mayora. Muñoz Araceli Barceló
Researcher
es.wikipedia.org CIFA / wiki / Mycorrhiza
How To Stop Him From Touching My Breasts
DEFINE "FOOD SAFETY.
What is it?
From the origins of humanity, the food was produced to meet the biological needs of individuals expressed in their communities reduced.
urban concentration, the increase in world population and the simultaneous increase in demand caused it to apply appropriate technologies to achieve a large-scale processing.
In today's world, and as a result of all these phenomena, the consumer gets less and less proportion of their food directly from natural sources (farms, orchards, sea, etc.). In most cases the food reaches the consumer after having covered a number of changes and transformations. In all aspects of the food chain - production, preparation, sale and consumption-should apply the concept of food safety which is a key factor in ensuring the health of the population.
According to the established by the Codex Alimentarius, food safety is the assurance that food will not cause harm to the consumer.
When it is prepared or eaten according to its intended use. Food is the main source of exposure to pathogens, both chemical and biological (viruses, parasites and bacteria), to which nobody is immune, not in developing countries or developed countries. When contaminated unacceptable levels of pathogens and chemical pollutants or other hazardous characteristics, involve substantial risks to human health consumers, and represent major economic burdens for the various communities and nations. The safety issue is very broad, also refers to chemical contaminants in foods, foods produced by modern biotechnology, microbiological risk assessment, and publications and documents.
Saturday, April 11, 2009
What Store Can I Buy Fake Braces From?
COCONUT LETHAL YELLOW
Free Trade Agreement between the United States, Central America and the Dominican Republic
"CAFTA" redirects here. For other meanings see CAFTA (disambiguation).
The Area is formed by CAFTA countries: Costa Rica, El Salvador, United States, Dominican Republic Guatemala, Honduras and Nicaragua.
The DR-CAFTA (Dominican Republic-Central America Free Trade Agreement, in English) or FTA (Free Trade Agreement-Dominican Republic-Central United States, in Castilian) is a treaty that seeks to create a free trade area between the signatory countries. Makes permanent the benefits for 80% of products offered by Central Basin Initiative (CBI), covering trade volume dólares.1 thirty billion consists of twenty chapters, each divided into articles.
The negotiation, signing and ratification of the treaty was carried out in different conditions, tailored to the characteristics and social and political context of each State party, the process began in 2003 for all countries except the Dominican Republic, namely the adoption of the text for all countries involved, in 2004 and came into force on different dates for each country since 2006. On the other hand, considering that the industry is fundamental to the treaty provisions concerning the business deal, it is important to address elements such as tariffs, customs motion, origin of products and the internal rules for freight traffic. As a complement, the CAFTA legislation dealing with aspects of hygienic production and protection the environment, respect for intellectual property rights and public and private investment, and any labor law in the States of the CAFTA area. It also specifies the mechanisms to settle disputes and to establish mutually agreed standards.
Despite the approval of the Contracting States, CAFTA has received many critical political and economic, opening a wide debate about the balance between benefits and disadvantages of the treaty provides.
Contents [hide] 1 Purpose
• Negotiations and signing • 2 • 3 Content
treaty or 3.1 Provisions concerning trade Tariff Treatment
3.1.1 3.1.2 Rules of Origin Customs Administration
3.1.3 3.1.4 Technical Barriers to Trade
or 3.2 Sanitary and phytosanitary
or 3.3 Investment 3.3.1 Procurement
or 3.4 Intellectual Property Rights 3.4.1 Products
3.4.2 source
Patent Provisions
or 3.5 or 3.6
labor law provisions on environmental law or 3.7
Dispute
• 4 or 4.1 Ratification Ratification Costa Ratification or 4.2
Rica in El Salvador
or 4.3 Ratification States Ratification United
or 4.4 or 4.5
in Guatemala in Honduras
Ratification Ratification or 4.6 or 4.7 in Nicaragua
Ratification in the Dominican Republic Reviews
• 5 • 6 See also 7 References
• Bibliography • 8 • 9 External links
Objectives [edit]
CAFTA's main purpose is to encourage expansion and diversification of trade in the region, eliminate obstacles to trade and facilitate cross-border movement of goods and services, promote fair competition in the free trade area, increase substantial opportunities investment and enforce property rights intelectual.2 Since it is officially stated objectives, it should be noted that compliance is subject to various factors including political will of governments, economic conditions of countries and joint application of the standards set by the treaty.
The treaty does not fall in contravention of previous regional agreements, so that the Central American integration process is not affected. However, any action taken as a region should be subject to the provisions of NAFTA, which puts the treaty over the new regional arrangements on
integración.2 Negotiations and signature [edit]
was negotiated on the basis of principles agreed upon by the parties. Apart from the rules of respect, there were three main elements to consider, any agreement made as a result of CAFTA, should be fully respectful of the constitutions of each country, seeking consistency of national law with the treaty, was considered the Central American countries merchants, as a single negotiating party, pretending to negotiate jointly, also agreed that it could only be implemented if the CAFTA negotiations had been completed, so that the interim agreements would not be valid alguna.3
Negotiations began in January 2003 and agreement was reached with El Salvador, Guatemala, Honduras and Nicaragua on December 17, 2003, and with Costa Rica on January 25, 2004. That same month, began negotiations with the Dominican Republic. On May 28, 2004, the Ministers of Commerce (Robert Zoellick) and Costa Rica (Alberto Trejos), the Salvadoran economy ministers (Miguel Angel Lacayo) and Guatemala (Marcio Cuevas), the Minister of Industry and Trade of Honduras (Norman García ) and the Ministry of Development, Industry and Commerce of Nicaragua (Mario Arana) signed the document in the building of the Organization of Americanos.4 A second ceremony for adopting the text with the Secretariat of State for Industry and Commerce of the Dominican Republic, Sonia Guzman, took place on August 5, 2004.5 On the other hand, although Panama is a Central American country, not negotiate with the rest of the region the treaty, making it uniquely United States, thus not included in the content of the treaty
CAFTA.6 [
The treaty consists of twenty chapters, each divided into articles. Its structure, allows the separate handling of various issues relating to free trade, regulating specialized way each item. Provisions concerning trade
Trade is the cornerstone that underpins integration economic, in the case of CAFTA, the agreement deals extensively on issues relating to commercial treatment in all areas involved. Regulations adopted by the countries is based on unifying the criteria for each state, leading to convention rules and procedures that are subject to exporters and importers of various products. The spirit of the treaty has been on equal treatment of goods and services originating in the CAFTA region, leaving aside the state protectionism on sectors of the economy.
tariff treatment [
Some agricultural products affected by CAFTA.
access to goods is based on a reduction process tariff, ie the goods entering tax-exempt exportación.7 Since Central American countries lack adequate conditions to compete with U.S. producers, the treaty provides for a grace period, which is tax deductible for some tariff escalation products. Since the product list is extensive in that situation, the explanation can be summarized in two points: each product is governed by ad hoc conditions, ie there is no uniform treatment even among signatory countries, so that the Salvadoran sugar be treated differently Honduran sugar, for ejemplo.8 addition, a second consideration respect, which is called the Special Agricultural Safeguard (SAE), which can be applied only by Central American countries except dairy products and peanuts, is the ability to levy additional tariffs on imported products before 2014, exceeding the productive capacity of the Rules of Origin
nacionales.7 [
The rules of origin are to determine the country to which he attributed the manufacture of a product, with the aim of verifying whether or not the subject of application tariff reduction under the tratado.7 A product is considered as originating in the region in the event of goods wholly obtained or produced entirely in the territory of one or more parties, it has been produced from originating materials and inputs or production of goods or inputs from non-originating materials, provided it is shown that the product contains at least 45% originaria.9 matter must consider that there are exceptions to the rule, allowing imports of some products such as footwear, pencils and iron without complying with the rules of origin. All products subject to this regime benefit from the elimination of tariffs, as provided in the Customs Administration
tratado.9 [
The treaty requires signatory states are required to publish its customs laws by means Legal and online, making available counseling offices to respecto.10 provides that States are obliged to ensure transparency in customs offices, not with any technical obstacle to the free passage of goods. The treaty requires that customs procedures are simple and fast, removing the ability to perform state inspections at mercancías.10 mass that is available all information about the registered goods at customs has confidential, 10 which restricts tools criminal and commercial research. In the event of any violations of national laws, the treaty left free to States for administrative or criminal act against those who break the law.10
Technical barriers to trade [
With CAFTA, the state loses to some extent the right to establish rules that restrict the movement of goods across its borders, unable to impose taxes or regulations to stop tax revenues or competitive advantages to producers nacionales.11 In this regard, CAFTA requires states to provide the maximum trade channels.
States parties should appoint a group of verifiers, which permanently ensure that the rules of trade affected not fulfilled. While the resolutions are not binding in this regard, States undertake to establish consensus on the measures adopten.11 The creation of state standards is not limited to national entities or persons, as the treaty requires states to all interesados.11 consensual
Sanitary and Phytosanitary Measures [
Cheese is one of the most subject to sanitary and phytosanitary checks.
The treaty requires assurances from the producers on the sanitary quality of products. Verifiable production processes and products rights.12 In the case, conflicts over this issue be settled by a committee of special conflict resolution, consisting of representatives of all parties, joining more than thirty days after login effect in each country.12 The committee extends its functions to the verification measures, advice and training, advice, mediation and resolution of conflictos.12 Each country is free to appoint their representatives, who have no major requirements to meet with national requirements. Investment
[
The chapter on investment is about the treatment that each state party will give investors of the other signatories of the treaty. In this regard, the central axis is equal treatment for all investors. The treaty clearly states in Chapter 10 that states are obliged to give such favorable conditions for foreigners and their nacionales.13 In this sense, we can say that the spirit of the treaty is to maintain the homogeneity of competencia.13 On the other hand, requires states to give all investors the minimum conditions under international law, providing security of property and support for their investment. While the treaty does not detail the specification, specify who will be the customary terms that constrain the behavior of
Estados.13 If there is a dispute, the foreign investor will have exactly the same rights as national depriving any preference, patronage, protection or choice of legal advice by the State of its nationals exclusively. There will be no expropriation of movable or immovable property by foreign investors, except for the cases provided for in the treaty, provided that the same treatment as nationals, in any case, compensation must be expressed and without protestas.13
A point relevant treaty provides that, in any way, investors are required to take out of the country of destination, in this sense, the treaty in no way guarantees that the increase in investment will be an encouragement to the national employment growth. differences between the state and investors are mainly settled by arbitration, leaving the courts as a last opción.13
Procurement
In public procurement, ie, services that provide domestic or foreign investors to the State, the CAFTA states that are guaranteed by foreign businessmen at least as favorable treatment given to the nationals. In this regard, a national company has no advantage in a competitive tender, being forced to compete under the same rules to their state counterparts unnatural contratante.14
In this regard, states are required to publish openly calls tender, by knowing the rules before the start of the competition. Prohibits any technical condition that is intended to benefit employers programs, such as the number of employees nacionales.14
The treaty establishes procedures for public procurement, abolishing all relevant national provisions, the procedures are uniform in the region, so any company will know the rules even if you invest outside the borders of his State of origen.14
The treaty guarantees the confidentiality of corporate information and requires transparency in bureaucratic processes, giving the State the power to suspend the ability to participate in bidding, those companies caught in acts
ilegales.14
intellectual property rights in the field of copyright and industrial property The treaty obliges states to accede to or ratify a number of conventions and treaties on the subject, draining discussion. Nothing can be above national treaties to ratify, so that the CAFTA establishes a new unified legal regime for partes.15 article seeks primarily to protect owners of intellectual property of products, provided these are duly registered. In terms of brands, the treaty provides that the owners of the same are treated under the same conditions as nationals, which means to exercise their rights on anyone who violates them.
The chapter on the subject, establishes the concept of "test data", which are those concerning to tests and experiments demonstrating the performance and effectiveness of pharmaceuticals and agrochemicals. The law protects the owners thereof for a period of five years to ten years for pharmaceuticals and agrochemicals. In this regard, no drug is patented in itself, so that any laboratory can produce the same, if proven by their own means that the formula complies with the sanitary requirements set by law. States are free to deny the protection of the test data in the event that either public domain or in emergency nacional.15 registration marks should be publicly established in each state, giving same facilities and protections to nationals and foreigners. Home products
The pupusas were a product in controversy regarding its patent in the end, the rule was not enforced.
are considered "products of" those whose conditions of production can only occur in a particular country, ensuring contracting calidad.15 CAFTA No country entered any product so original, so this rule is reserved for when there is a case. The scheme provides that the country will have the exclusive right to produce, to ensure quality. Patent
respect, the treaty provides that any natural CAFTA area patentable inventions or discoveries of their own, being protected by the law as if national. You can not patent anything for public use, known prior to the investigation or previously patented by another persona.15 Any violation of copyright can lead to administrative or judicial responsibilities.
provisions on labor law requirements increases CAFTA in employment law for Central American countries, forcing them to raise standards for the benefit of workers. It is established that there is no difference in treatment between natural and extranjeros.16 No provisions on compulsory hiring local or foreign, minimum fee for companies or distinctions between them.
On the other hand, the treaty obliges the state to have enough inspectors, in charge of verifying compliance with international agreements on laboral.16 should be noted that CAFTA does not make provisions for or at the expense of workers, simply requires States to comply with regulations and commitments previously.
CAFTA provides that any reduction in industrial action in terms of attracting investment is illegal and subject to sanción.16
environmental law provisions
factory polluting the environment, which is regulated by CAFTA.
CAFTA is the first trade agreement which devotes a chapter to the field of environmental protection, 8 and in the workplace, it provides no measures or procedures, rather is dedicated to compel states to enforce national laws and international existentes.17 and conductive line of the chapter is based to strengthen national environmental laws, which are defined in Article 17.13 as law or regulation
Party, or provisions thereof, the primary purpose of environmental protection or prevention of a danger to life or human health, animal or plant by:
• prevention, reduction or control of the release, discharge or emission of pollutants environmental;
• control of chemicals, substances, materials and environmentally hazardous or toxic waste and the dissemination of information related thereto, or
• protection or conservation of wildlife, including endangered species, their habitat and natural areas under special protection. 17.13-CAFTA Art
The treaty establishes procedures for punishing offenders regardless of nationality, sanctions should be attached to international law and comparable to the rest of the CAFTA area. Moreover, CAFTA provides that the State will encourage companies to implement measures to protect the environment are above the minimum levels deseados.17
The treaty requires states to participate in mutual cooperation in environmental matters, therefore, parallel to the CAFTA, created an Environmental Cooperation Agreement between the United States, Central America and Dominicana.18 Republic CAFTA provides that any reduction of environmental measures in order to attract investment is illegal and subject to sanciones.17
Dispute
When any dispute arises between States (never between company and State), are recognized methods good offices, mediation or conciliation. Except in extreme cases of disagreement, to accept the appointment of árbitros.19 means that participation Referees will only if a prolonged time of no progress in the conflict, ie, the parties should recognize the stalled negotiations and agreement to appoint an arbitration board. The procedure for appointing the arbitration is governed in CAFTA, which states that the parties shall select three arbitrators, including a president be in agreement, in the case of no agreement, be settled by sorteo.19
The CAFTA guarantees due process, confidentiality, non-retroactivity and the opportunity to defend and respond. Once an award, the parties must abide by it without protest, to do so, will be suspended benefits relating to CAFTA, but not their obligaciones.19
According Ratification of the Vienna Convention on the Law of Treaties of 1969 and in force since 1980, the text of a treaty is adopted when two-thirds of the attendees to the negotiation agree, in the case of CAFTA, all parties agreed to adopt the texto.20 after adopting the text, the Convention states that must be initialed by the plenipotentiaries of the States, 20 CAFTA was signed by all States businessmen. Following that, the national congresses of each State should ratify the treaty according to the laws and national interests, after which the Foreign Ministry spent to process for entry into vigor.20 All signatory countries have ratified the Ratification
tratado.21 in Costa Rica
Main article:
CAFTA in Costa Rica Costa Rica is the only signatory to the treaty which submitted its ratification to a referendum , which was conducted with support from the Supreme Court Elecciones.22 23 The referendum was held on October 7, 2007, resulting in a 51.62% in favor and 48.38% against, being bound by more than 40% of electorate exerted by the sufragio.24 Given these results, the CAFTA was ratified.
In this respect, labor groups and groups opposed to the CAFTA developed a campaign to prevent that people vote yes, including sharing information and mobilizing people to protestas.23 However, there were also groups that supported the ratification of the treaty by massive publicity and activities concentración.25
As part of the campaign by critics treaty, constitutional challenges were filed before the Supreme Court of Costa Rica, in parallel with this, opposition MPs called for an inquiry to the highest judicial institution, which issued by the Constitutional Chamber of Resolution 2007-09469 July 3, 2007, in which he states that there are points that violate the constitution of the Republic for approval
CAFTA.26 necessary implementing laws were passed and the treaty entered into force in Costa Rica on January 1st 2009. Ratification
in El Salvador
Main article: CAFTA in El Salvador
El Salvador was the first state to ratify CAFTA, on the morning of December 17, 2004 in the Blue Room of the Legislative Assembly, 27 with the support of all political forces except the Farabundo Marti National Liberation Front. After ratification, the respective deposit was made at the Organization of American States on 28 February 2006.28
Several trade unions and opposition groups tried organized street protests with the slogan to prevent the treaty was ratified, some protests were counted with the presence and support of the leaders of some opposition political parties. The balance of the demonstrations was in several episodes of violence that left detainees and wounded. All events took place under the observation of the Ombudsman for the Defence of Rights Ratification Humanos.29 U.S.
Main article: U.S. CAFTA
Upon notification by President George Bush in compliance with the Trade Act of 2002 , 30 the United States Senate ratified the June 30, 2005 CAFTA, with fifty-four and forty votes and five against. That ratification was engaged in a debate about the feasibility of implementing the treaty, to consider some senators that it would bring little benefit to the country norteamericano.31
Ratification in Guatemala [Main article: CAFTA in Guatemala Guatemalan Congress ratified
10 March 2005 the treaty by the Decree 31 to 2005.32 with one hundred twenty-six and twelve votes against, giving quality of national emergency by holding it a priority issue in the treatment legislativo.33 was the third country to ratify, after El Salvador and Honduras. On March 9, the treaty was presented to the Legislature by the commission chairman of Economy and Foreign Trade, Mariano Rayo, without having had any discussion or study of the proposal, the treaty was ratified and notified the President of the Republic, for their respective publicación.33 The approval was made possible by changing the position of the bench the National Union of Hope, who gave their votes.
Members who voted against ratification, arguing that the deal was negotiated behind
population ... only defends the interests of a small group of businessmen ... and the government has been reluctant to report the true content, purpose and impact Víctor
Sales, deputy of the National Revolutionary Unity party
Guatemalteca33 Days before the introduction of the proposal, various organizations and institutions presented their show of rejection of the treaty. Thus, on 1 March, accompanied by an integrated street protest by several unions, the Consejo Superior Universitario de la Universidad de San Carlos TV issued a statement expressing their opposition to the ratification of the treaty without prior consultation. Two days later, the rector of the university formally asked the House to be a query to include the entire ciudadanía.32 On 6 March, the Episcopal Conference of Guatemala ruled against the treaty.
The day the proposal was submitted parliament, and the date of approval of the same, there were violent protests around the Congress and the U.S. Embassy, \u200b\u200b32 requiring the ratification of the treaty was submitted to a referendum, which dictate the popular will. 34 The balance of the protests was a police officer injured and a man arrested for the crime of Ratification
públicos.33 disorders in Honduras
Main article: CAFTA in Honduras Honduran
's parliament ratified the treaty on March 3, 2005 with votes Four of the five legislative seats, becoming the second country to ratify the treaty. Following the adoption of the decree, groups unions and opposition to the treaty entered the session hall of the congress, forcing security forces to quell the protesta.1 In general, several organizations took to the streets and protested against what they contend is a step in the impoverishment of the country. Despite the many demonstrations, the police reported no episodes of Ratification in Nicaragua
violencia.35
Main article: CAFTA in Nicaragua Nicaraguan
Congress passed the night of October 10, 2005 ratification of CAFTA, with forty-nine votes favor and thirty-six against, and three abstenciones.36 The legislative proposal was introduced by the minister of trade, Azucena Castillo. Discussion among the parliamentary groups was extensive and led to the adoption of the treaty by a simple majority. The fundamental argument of the opposition was that the treaty would lead to "increased the gap between rich and poor" .37 The main leftist party in Nicaragua, the Sandinista National Liberation Front, led many street protests in order to halting the ratification of the treaty, arguing that the provisions to take the call favoring only upper-class country. Despite the number of people confronted the police, there were no incidents of violencia.38 With the change of party in the head of state, President Daniel Ortega has spoken against the decision taken by his predecessor, strongly criticizing the treaty is already in vigor.39
Ratification in the Dominican Republic [edit]
Main article: CAFTA in the Dominican Republic Dominican
Congress sought to bring national laws to the operation of CAFTA waiting to unanimously ratify until the legislation was compatible. So in 2005, approved a measure would come into force the treaty a year siguiente.40 sectors protests opposing the treaty took place in Santo Domingo, including hunger strikes and stoppage of traffic. Although there were no acts of violence, the authorities seek to arrest leaders of
protestas.41
Graffiti Reviews
against CAFTA in Costa Rica.
In general, the treaty has generated much political opposition and civil society in the signatory countries. Institutions such as ECLAC indicated that CAFTA does not bring real solutions to the problems of Central America, with only a tool económica.42 Even in the U.S. Congress there were serious criticisms for ratification, exceeding the minimum number of votes. Some U.S. lawmakers claim that CAFTA will only serve to increase unemployment and crime in countries centroamericanos.43 The main opponents of CAFTA Central American businesses say it is not competitive opportunities with the U.S., since the volumes of capital are heterogeneous, while CAFTA requires treating them as if they were homogeneous.
is also critical that American products will be severely affected in sales volumes, as they can not compete with U.S. prices and quality, bankrupting many businesses, farmers and small producers. Critics claim that unemployment will grow rapidly approaching to the close of business, considering that the treaty does not guarantee employment or even a favorable environment for foreign investment. They fear damage the health of the population genetically modified products, the destruction of the environment by excessive industrial production and the lack of drug production rights on the basis of 45
patentes.44
Free Trade Agreement between the United States, Central America and the Dominican Republic
"CAFTA" redirects here. For other meanings see CAFTA (disambiguation).
The Area is formed by CAFTA countries: Costa Rica, El Salvador, United States, Dominican Republic Guatemala, Honduras and Nicaragua.
The DR-CAFTA (Dominican Republic-Central America Free Trade Agreement, in English) or FTA (Free Trade Agreement-Dominican Republic-Central United States, in Castilian) is a treaty that seeks to create a free trade area between the signatory countries. Makes permanent the benefits for 80% of products offered by Central Basin Initiative (CBI), covering trade volume dólares.1 thirty billion consists of twenty chapters, each divided into articles.
The negotiation, signing and ratification of the treaty was carried out in different conditions, tailored to the characteristics and social and political context of each State party, the process began in 2003 for all countries except the Dominican Republic, namely the adoption of the text for all countries involved, in 2004 and came into force on different dates for each country since 2006. On the other hand, considering that the industry is fundamental to the treaty provisions concerning the business deal, it is important to address elements such as tariffs, customs motion, origin of products and the internal rules for freight traffic. As a complement, the CAFTA legislation dealing with aspects of hygienic production and protection the environment, respect for intellectual property rights and public and private investment, and any labor law in the States of the CAFTA area. It also specifies the mechanisms to settle disputes and to establish mutually agreed standards.
Despite the approval of the Contracting States, CAFTA has received many critical political and economic, opening a wide debate about the balance between benefits and disadvantages of the treaty provides.
Contents [hide] 1 Purpose
• Negotiations and signing • 2 • 3 Content
treaty or 3.1 Provisions concerning trade Tariff Treatment
3.1.1 3.1.2 Rules of Origin Customs Administration
3.1.3 3.1.4 Technical Barriers to Trade
or 3.2 Sanitary and phytosanitary
or 3.3 Investment 3.3.1 Procurement
or 3.4 Intellectual Property Rights 3.4.1 Products
3.4.2 source
Patent Provisions
or 3.5 or 3.6
labor law provisions on environmental law or 3.7
Dispute
• 4 or 4.1 Ratification Ratification Costa Ratification or 4.2
Rica in El Salvador
or 4.3 Ratification States Ratification United
or 4.4 or 4.5
in Guatemala in Honduras
Ratification Ratification or 4.6 or 4.7 in Nicaragua
Ratification in the Dominican Republic Reviews
• 5 • 6 See also 7 References
• Bibliography • 8 • 9 External links
Objectives [edit]
CAFTA's main purpose is to encourage expansion and diversification of trade in the region, eliminate obstacles to trade and facilitate cross-border movement of goods and services, promote fair competition in the free trade area, increase substantial opportunities investment and enforce property rights intelectual.2 Since it is officially stated objectives, it should be noted that compliance is subject to various factors including political will of governments, economic conditions of countries and joint application of the standards set by the treaty.
The treaty does not fall in contravention of previous regional agreements, so that the Central American integration process is not affected. However, any action taken as a region should be subject to the provisions of NAFTA, which puts the treaty over the new regional arrangements on
integración.2 Negotiations and signature [edit]
was negotiated on the basis of principles agreed upon by the parties. Apart from the rules of respect, there were three main elements to consider, any agreement made as a result of CAFTA, should be fully respectful of the constitutions of each country, seeking consistency of national law with the treaty, was considered the Central American countries merchants, as a single negotiating party, pretending to negotiate jointly, also agreed that it could only be implemented if the CAFTA negotiations had been completed, so that the interim agreements would not be valid alguna.3
Negotiations began in January 2003 and agreement was reached with El Salvador, Guatemala, Honduras and Nicaragua on December 17, 2003, and with Costa Rica on January 25, 2004. That same month, began negotiations with the Dominican Republic. On May 28, 2004, the Ministers of Commerce (Robert Zoellick) and Costa Rica (Alberto Trejos), the Salvadoran economy ministers (Miguel Angel Lacayo) and Guatemala (Marcio Cuevas), the Minister of Industry and Trade of Honduras (Norman García ) and the Ministry of Development, Industry and Commerce of Nicaragua (Mario Arana) signed the document in the building of the Organization of Americanos.4 A second ceremony for adopting the text with the Secretariat of State for Industry and Commerce of the Dominican Republic, Sonia Guzman, took place on August 5, 2004.5 On the other hand, although Panama is a Central American country, not negotiate with the rest of the region the treaty, making it uniquely United States, thus not included in the content of the treaty
CAFTA.6 [
The treaty consists of twenty chapters, each divided into articles. Its structure, allows the separate handling of various issues relating to free trade, regulating specialized way each item. Provisions concerning trade
Trade is the cornerstone that underpins integration economic, in the case of CAFTA, the agreement deals extensively on issues relating to commercial treatment in all areas involved. Regulations adopted by the countries is based on unifying the criteria for each state, leading to convention rules and procedures that are subject to exporters and importers of various products. The spirit of the treaty has been on equal treatment of goods and services originating in the CAFTA region, leaving aside the state protectionism on sectors of the economy.
tariff treatment [
Some agricultural products affected by CAFTA.
access to goods is based on a reduction process tariff, ie the goods entering tax-exempt exportación.7 Since Central American countries lack adequate conditions to compete with U.S. producers, the treaty provides for a grace period, which is tax deductible for some tariff escalation products. Since the product list is extensive in that situation, the explanation can be summarized in two points: each product is governed by ad hoc conditions, ie there is no uniform treatment even among signatory countries, so that the Salvadoran sugar be treated differently Honduran sugar, for ejemplo.8 addition, a second consideration respect, which is called the Special Agricultural Safeguard (SAE), which can be applied only by Central American countries except dairy products and peanuts, is the ability to levy additional tariffs on imported products before 2014, exceeding the productive capacity of the Rules of Origin
nacionales.7 [
The rules of origin are to determine the country to which he attributed the manufacture of a product, with the aim of verifying whether or not the subject of application tariff reduction under the tratado.7 A product is considered as originating in the region in the event of goods wholly obtained or produced entirely in the territory of one or more parties, it has been produced from originating materials and inputs or production of goods or inputs from non-originating materials, provided it is shown that the product contains at least 45% originaria.9 matter must consider that there are exceptions to the rule, allowing imports of some products such as footwear, pencils and iron without complying with the rules of origin. All products subject to this regime benefit from the elimination of tariffs, as provided in the Customs Administration
tratado.9 [
The treaty requires signatory states are required to publish its customs laws by means Legal and online, making available counseling offices to respecto.10 provides that States are obliged to ensure transparency in customs offices, not with any technical obstacle to the free passage of goods. The treaty requires that customs procedures are simple and fast, removing the ability to perform state inspections at mercancías.10 mass that is available all information about the registered goods at customs has confidential, 10 which restricts tools criminal and commercial research. In the event of any violations of national laws, the treaty left free to States for administrative or criminal act against those who break the law.10
Technical barriers to trade [
With CAFTA, the state loses to some extent the right to establish rules that restrict the movement of goods across its borders, unable to impose taxes or regulations to stop tax revenues or competitive advantages to producers nacionales.11 In this regard, CAFTA requires states to provide the maximum trade channels.
States parties should appoint a group of verifiers, which permanently ensure that the rules of trade affected not fulfilled. While the resolutions are not binding in this regard, States undertake to establish consensus on the measures adopten.11 The creation of state standards is not limited to national entities or persons, as the treaty requires states to all interesados.11 consensual
Sanitary and Phytosanitary Measures [
Cheese is one of the most subject to sanitary and phytosanitary checks.
The treaty requires assurances from the producers on the sanitary quality of products. Verifiable production processes and products rights.12 In the case, conflicts over this issue be settled by a committee of special conflict resolution, consisting of representatives of all parties, joining more than thirty days after login effect in each country.12 The committee extends its functions to the verification measures, advice and training, advice, mediation and resolution of conflictos.12 Each country is free to appoint their representatives, who have no major requirements to meet with national requirements. Investment
[
The chapter on investment is about the treatment that each state party will give investors of the other signatories of the treaty. In this regard, the central axis is equal treatment for all investors. The treaty clearly states in Chapter 10 that states are obliged to give such favorable conditions for foreigners and their nacionales.13 In this sense, we can say that the spirit of the treaty is to maintain the homogeneity of competencia.13 On the other hand, requires states to give all investors the minimum conditions under international law, providing security of property and support for their investment. While the treaty does not detail the specification, specify who will be the customary terms that constrain the behavior of
Estados.13 If there is a dispute, the foreign investor will have exactly the same rights as national depriving any preference, patronage, protection or choice of legal advice by the State of its nationals exclusively. There will be no expropriation of movable or immovable property by foreign investors, except for the cases provided for in the treaty, provided that the same treatment as nationals, in any case, compensation must be expressed and without protestas.13
A point relevant treaty provides that, in any way, investors are required to take out of the country of destination, in this sense, the treaty in no way guarantees that the increase in investment will be an encouragement to the national employment growth. differences between the state and investors are mainly settled by arbitration, leaving the courts as a last opción.13
Procurement
In public procurement, ie, services that provide domestic or foreign investors to the State, the CAFTA states that are guaranteed by foreign businessmen at least as favorable treatment given to the nationals. In this regard, a national company has no advantage in a competitive tender, being forced to compete under the same rules to their state counterparts unnatural contratante.14
In this regard, states are required to publish openly calls tender, by knowing the rules before the start of the competition. Prohibits any technical condition that is intended to benefit employers programs, such as the number of employees nacionales.14
The treaty establishes procedures for public procurement, abolishing all relevant national provisions, the procedures are uniform in the region, so any company will know the rules even if you invest outside the borders of his State of origen.14
The treaty guarantees the confidentiality of corporate information and requires transparency in bureaucratic processes, giving the State the power to suspend the ability to participate in bidding, those companies caught in acts
ilegales.14
intellectual property rights in the field of copyright and industrial property The treaty obliges states to accede to or ratify a number of conventions and treaties on the subject, draining discussion. Nothing can be above national treaties to ratify, so that the CAFTA establishes a new unified legal regime for partes.15 article seeks primarily to protect owners of intellectual property of products, provided these are duly registered. In terms of brands, the treaty provides that the owners of the same are treated under the same conditions as nationals, which means to exercise their rights on anyone who violates them.
The chapter on the subject, establishes the concept of "test data", which are those concerning to tests and experiments demonstrating the performance and effectiveness of pharmaceuticals and agrochemicals. The law protects the owners thereof for a period of five years to ten years for pharmaceuticals and agrochemicals. In this regard, no drug is patented in itself, so that any laboratory can produce the same, if proven by their own means that the formula complies with the sanitary requirements set by law. States are free to deny the protection of the test data in the event that either public domain or in emergency nacional.15 registration marks should be publicly established in each state, giving same facilities and protections to nationals and foreigners. Home products
The pupusas were a product in controversy regarding its patent in the end, the rule was not enforced.
are considered "products of" those whose conditions of production can only occur in a particular country, ensuring contracting calidad.15 CAFTA No country entered any product so original, so this rule is reserved for when there is a case. The scheme provides that the country will have the exclusive right to produce, to ensure quality. Patent
respect, the treaty provides that any natural CAFTA area patentable inventions or discoveries of their own, being protected by the law as if national. You can not patent anything for public use, known prior to the investigation or previously patented by another persona.15 Any violation of copyright can lead to administrative or judicial responsibilities.
provisions on labor law requirements increases CAFTA in employment law for Central American countries, forcing them to raise standards for the benefit of workers. It is established that there is no difference in treatment between natural and extranjeros.16 No provisions on compulsory hiring local or foreign, minimum fee for companies or distinctions between them.
On the other hand, the treaty obliges the state to have enough inspectors, in charge of verifying compliance with international agreements on laboral.16 should be noted that CAFTA does not make provisions for or at the expense of workers, simply requires States to comply with regulations and commitments previously.
CAFTA provides that any reduction in industrial action in terms of attracting investment is illegal and subject to sanción.16
environmental law provisions
factory polluting the environment, which is regulated by CAFTA.
CAFTA is the first trade agreement which devotes a chapter to the field of environmental protection, 8 and in the workplace, it provides no measures or procedures, rather is dedicated to compel states to enforce national laws and international existentes.17 and conductive line of the chapter is based to strengthen national environmental laws, which are defined in Article 17.13 as law or regulation
Party, or provisions thereof, the primary purpose of environmental protection or prevention of a danger to life or human health, animal or plant by:
• prevention, reduction or control of the release, discharge or emission of pollutants environmental;
• control of chemicals, substances, materials and environmentally hazardous or toxic waste and the dissemination of information related thereto, or
• protection or conservation of wildlife, including endangered species, their habitat and natural areas under special protection. 17.13-CAFTA Art
The treaty establishes procedures for punishing offenders regardless of nationality, sanctions should be attached to international law and comparable to the rest of the CAFTA area. Moreover, CAFTA provides that the State will encourage companies to implement measures to protect the environment are above the minimum levels deseados.17
The treaty requires states to participate in mutual cooperation in environmental matters, therefore, parallel to the CAFTA, created an Environmental Cooperation Agreement between the United States, Central America and Dominicana.18 Republic CAFTA provides that any reduction of environmental measures in order to attract investment is illegal and subject to sanciones.17
Dispute
When any dispute arises between States (never between company and State), are recognized methods good offices, mediation or conciliation. Except in extreme cases of disagreement, to accept the appointment of árbitros.19 means that participation Referees will only if a prolonged time of no progress in the conflict, ie, the parties should recognize the stalled negotiations and agreement to appoint an arbitration board. The procedure for appointing the arbitration is governed in CAFTA, which states that the parties shall select three arbitrators, including a president be in agreement, in the case of no agreement, be settled by sorteo.19
The CAFTA guarantees due process, confidentiality, non-retroactivity and the opportunity to defend and respond. Once an award, the parties must abide by it without protest, to do so, will be suspended benefits relating to CAFTA, but not their obligaciones.19
According Ratification of the Vienna Convention on the Law of Treaties of 1969 and in force since 1980, the text of a treaty is adopted when two-thirds of the attendees to the negotiation agree, in the case of CAFTA, all parties agreed to adopt the texto.20 after adopting the text, the Convention states that must be initialed by the plenipotentiaries of the States, 20 CAFTA was signed by all States businessmen. Following that, the national congresses of each State should ratify the treaty according to the laws and national interests, after which the Foreign Ministry spent to process for entry into vigor.20 All signatory countries have ratified the Ratification
tratado.21 in Costa Rica
Main article:
CAFTA in Costa Rica Costa Rica is the only signatory to the treaty which submitted its ratification to a referendum , which was conducted with support from the Supreme Court Elecciones.22 23 The referendum was held on October 7, 2007, resulting in a 51.62% in favor and 48.38% against, being bound by more than 40% of electorate exerted by the sufragio.24 Given these results, the CAFTA was ratified.
In this respect, labor groups and groups opposed to the CAFTA developed a campaign to prevent that people vote yes, including sharing information and mobilizing people to protestas.23 However, there were also groups that supported the ratification of the treaty by massive publicity and activities concentración.25
As part of the campaign by critics treaty, constitutional challenges were filed before the Supreme Court of Costa Rica, in parallel with this, opposition MPs called for an inquiry to the highest judicial institution, which issued by the Constitutional Chamber of Resolution 2007-09469 July 3, 2007, in which he states that there are points that violate the constitution of the Republic for approval
CAFTA.26 necessary implementing laws were passed and the treaty entered into force in Costa Rica on January 1st 2009. Ratification
in El Salvador
Main article: CAFTA in El Salvador
El Salvador was the first state to ratify CAFTA, on the morning of December 17, 2004 in the Blue Room of the Legislative Assembly, 27 with the support of all political forces except the Farabundo Marti National Liberation Front. After ratification, the respective deposit was made at the Organization of American States on 28 February 2006.28
Several trade unions and opposition groups tried organized street protests with the slogan to prevent the treaty was ratified, some protests were counted with the presence and support of the leaders of some opposition political parties. The balance of the demonstrations was in several episodes of violence that left detainees and wounded. All events took place under the observation of the Ombudsman for the Defence of Rights Ratification Humanos.29 U.S.
Main article: U.S. CAFTA
Upon notification by President George Bush in compliance with the Trade Act of 2002 , 30 the United States Senate ratified the June 30, 2005 CAFTA, with fifty-four and forty votes and five against. That ratification was engaged in a debate about the feasibility of implementing the treaty, to consider some senators that it would bring little benefit to the country norteamericano.31
Ratification in Guatemala [Main article: CAFTA in Guatemala Guatemalan Congress ratified
10 March 2005 the treaty by the Decree 31 to 2005.32 with one hundred twenty-six and twelve votes against, giving quality of national emergency by holding it a priority issue in the treatment legislativo.33 was the third country to ratify, after El Salvador and Honduras. On March 9, the treaty was presented to the Legislature by the commission chairman of Economy and Foreign Trade, Mariano Rayo, without having had any discussion or study of the proposal, the treaty was ratified and notified the President of the Republic, for their respective publicación.33 The approval was made possible by changing the position of the bench the National Union of Hope, who gave their votes.
Members who voted against ratification, arguing that the deal was negotiated behind
population ... only defends the interests of a small group of businessmen ... and the government has been reluctant to report the true content, purpose and impact Víctor
Sales, deputy of the National Revolutionary Unity party
Guatemalteca33 Days before the introduction of the proposal, various organizations and institutions presented their show of rejection of the treaty. Thus, on 1 March, accompanied by an integrated street protest by several unions, the Consejo Superior Universitario de la Universidad de San Carlos TV issued a statement expressing their opposition to the ratification of the treaty without prior consultation. Two days later, the rector of the university formally asked the House to be a query to include the entire ciudadanía.32 On 6 March, the Episcopal Conference of Guatemala ruled against the treaty.
The day the proposal was submitted parliament, and the date of approval of the same, there were violent protests around the Congress and the U.S. Embassy, \u200b\u200b32 requiring the ratification of the treaty was submitted to a referendum, which dictate the popular will. 34 The balance of the protests was a police officer injured and a man arrested for the crime of Ratification
públicos.33 disorders in Honduras
Main article: CAFTA in Honduras Honduran
's parliament ratified the treaty on March 3, 2005 with votes Four of the five legislative seats, becoming the second country to ratify the treaty. Following the adoption of the decree, groups unions and opposition to the treaty entered the session hall of the congress, forcing security forces to quell the protesta.1 In general, several organizations took to the streets and protested against what they contend is a step in the impoverishment of the country. Despite the many demonstrations, the police reported no episodes of Ratification in Nicaragua
violencia.35
Main article: CAFTA in Nicaragua Nicaraguan
Congress passed the night of October 10, 2005 ratification of CAFTA, with forty-nine votes favor and thirty-six against, and three abstenciones.36 The legislative proposal was introduced by the minister of trade, Azucena Castillo. Discussion among the parliamentary groups was extensive and led to the adoption of the treaty by a simple majority. The fundamental argument of the opposition was that the treaty would lead to "increased the gap between rich and poor" .37 The main leftist party in Nicaragua, the Sandinista National Liberation Front, led many street protests in order to halting the ratification of the treaty, arguing that the provisions to take the call favoring only upper-class country. Despite the number of people confronted the police, there were no incidents of violencia.38 With the change of party in the head of state, President Daniel Ortega has spoken against the decision taken by his predecessor, strongly criticizing the treaty is already in vigor.39
Ratification in the Dominican Republic [edit]
Main article: CAFTA in the Dominican Republic Dominican
Congress sought to bring national laws to the operation of CAFTA waiting to unanimously ratify until the legislation was compatible. So in 2005, approved a measure would come into force the treaty a year siguiente.40 sectors protests opposing the treaty took place in Santo Domingo, including hunger strikes and stoppage of traffic. Although there were no acts of violence, the authorities seek to arrest leaders of
protestas.41
Graffiti Reviews
against CAFTA in Costa Rica.
In general, the treaty has generated much political opposition and civil society in the signatory countries. Institutions such as ECLAC indicated that CAFTA does not bring real solutions to the problems of Central America, with only a tool económica.42 Even in the U.S. Congress there were serious criticisms for ratification, exceeding the minimum number of votes. Some U.S. lawmakers claim that CAFTA will only serve to increase unemployment and crime in countries centroamericanos.43 The main opponents of CAFTA Central American businesses say it is not competitive opportunities with the U.S., since the volumes of capital are heterogeneous, while CAFTA requires treating them as if they were homogeneous.
is also critical that American products will be severely affected in sales volumes, as they can not compete with U.S. prices and quality, bankrupting many businesses, farmers and small producers. Critics claim that unemployment will grow rapidly approaching to the close of business, considering that the treaty does not guarantee employment or even a favorable environment for foreign investment. They fear damage the health of the population genetically modified products, the destruction of the environment by excessive industrial production and the lack of drug production rights on the basis of 45
Cost To Laminate At Staples
CAFTA (ALC)
Introduction
coconut cultivation (Cocos nucifera L) is of economic, social and ecological to the Dominican Republic, which produces the currency, jobs and income, food and other products for local and international market. Besides coconut palms grace the beaches of the main tourist areas, providing its tropical beauty to the landscape, and therefore it which encourages tourists visit the country.
In Dominican Republic, the cultivated area is 22.491 ha. 359, 856 TAS. With an average annual production of 2,227, 872.8, units in the east and west coastal areas (Ministry of Agriculture, 2006). The production of fresh coconuts and products are intended for national and international markets, the latter generating U.S. $ 4, 553,143.00 Dollars (CEI-RD, 2005) by way of exports.
In 2006 the Dominican Republic was visited by 2, 612.256 tourists from different parts of the world, generating tourism input $ 3.750 and more than 300 thousand jobs (Martínez, 2006).
The coconut lethal yellowing disease (ALC) is considered the most devastating affects this crop, which has killed hundreds of thousands of palms in Key West, Florida, USA, from the Caribbean 1936.En has caused the disappearance of this crop, destroying millions of coconut palms in the last 40 years (OIRSA, 2002). CLA is caused by a phytoplasma, transmitted by the insect vector crudus Myndus Van Duzee, of the order Homoptera: Family Cixiidae. (Howard, 1983).
The ALC has been reported in the Dominican Republic several times since 1962, affecting domestic plantations in locations the province of Puerto Plata, Santiago and Dajabón (Hichez, 1971). Recently it was reported an outbreak in the town of Andrés, Boca Chica, Santo Domingo Province. (Martinez et al, 2007).
The detection of this disease is carried by the technique of reaction polymerase chain (PCR), which is a molecular technique that amplifies nucleic acid bands.
Because these pathogens can not grow invitro, recommended diagnostic technique using Chain Reaction (PCR), which is a molecular technique that amplifies nucleic acid bands. The PCR is recommended for detection of phytoplasmas and genetic characterization of these. Phytoplasma known races allow management to devise strategies to prevent the spread of disease and damage in commercial plantations.
I.
PROBLEM 1.1. Description and formulation of the problem.
The coconut lethal yellowing disease (ALC) has been reported in the Dominican Republic since 1962, affecting domestic plantations in the villages of the provinces of Puerto Plata, Santiago and Dajabón. In each occurrence of the disease have been implemented measures such as slashing and burning of diseased plants to prevent the rapid dispersion the same (Hichez, 1971).
The most recent occurrence of CLA was reported in 2006 in the east coast in the town of Andrés, Boca Chica, with a larger number of symptomatic plants, which reported outbreaks in previous years in the North, (Martinez, personal communication 2006).
This disease represents a potential threat to the production of coconuts in the Dominican Republic, as this has been reported as the most devastating crop of coconut palms; Initial symptoms are characterized by the premature fall of the fruits of all sizes, the lower leaves, intermediate and higher turn yellow, the heads and knives become necrotic, in the final stage all the leaves fall, leaving only the stem erect (Córdova, 1995). 1.2 OBJECTIVES
objective 1.2.1:
Diagnosis and characterization of strains of phytoplasmas coconut lethal yellowing.
1.2.2 Specific objectives
a) determine the presence of phytoplasma.
b) Genetic differentiation of phytoplasma strains in the country.
c) Establish the relationship of the phytoplasmas found in the Dominican Republic with other países.México, Honduras, Belize, Guatemala, USA (Florida), Jamaica, Cuba, Cayman and Haiti.
1.3 - Justification
For the Dominican Republic coconut cultivation is of great importance because it is used in agriculture and tourism, generating foreign exchange for the country as part of the export of the product and concept as tourism, attracted by the coconut.
The production of fresh coconuts, grated, dried, and products for the cosmetics industry, food and medicine is intended for local and international markets, both the United States, European Union, Canada, West Indies Children, Virgin Islands, Brazil, Puerto Rico, Haiti and others. Foreign exchange earnings in 2005, this concept was worth $ 2, 282, 987.80, USD. In 2004 and $ 4, 553,143.00, USD (CEI-RD, 2005). It is important to note that Nationwide thousands of families depend on this crop for their livelihood.
coconut palms beaches located in major tourist areas, provide the tropical beauty of the landscape, which encourages tourists visit. In 2006 the Dominican Republic was visited by 2,612,256 tourists from different parts of the world, generating tourism $ 3.750 input. More than 300 thousand. Jobs (Martínez, 2006).
business opportunities for The Dominican Republic is developing exports, as the nations of North America, Europe, South America and the Caribbean require greater amount of fresh coconut, processed and a wide variety of products. Today has resumed the use of coconut oil for culinary purposes, such as virgin coconut oil. In the United States and Europe, ½ liter sells for $ 16.00 and U.S. $ 15.75, respectively. This represents an opportunity to diversify production and increase foreign exchange earnings from this crop (CEI-RD 2006). 1:4
Diagnosis and Characterization
The diagnosis and understanding of the genetic diversity of the pathogen in the country help design management strategies to prevent the spread of disease and damage to areas of commercial production. So the knowledge of the races will help design management strategies to prevent the spread and damage to commercial plantations.
This research would provide the basis for the continuity of production and the benefits it provides to more than 1500 coconut farmers, processors, exporters and other members of the Dominican Republic in the production chain as well as foreign exchange earnings and employment sources both sectors and the preservation of the natural landscape that give the coconut palms to the country's beaches. Creating the basis for both sectors are more competitive and sustainable, especially in times of opening new markets.
1.4 Scope and limitations
1.4.1 Scope The study will include the towns of the North (Puerto Plata, Santiago, Dajabón) and Southeast (Andrés, Boca Chica) where the disease has been reported and in the production regions Northeast Commercial (Maria Trinidad Sanchez, Samana) and Eastern (Hato Mayor, El Seibo, La Altagracia, La Romana) in the country. Which has not been reported. and to ensure that these areas are free of the disease. 1.4.2 Limitations
To determine the sequence of base pairs of possible phytoplasma in the country, will be sent to international centers engaged in such activity, as in the Dominican Republic there are no possibilities for these techniques.
11. THEORETICAL FRAMEWORK 2.1 General
Cocos nucifera L.
(Belonging to the family Arecaceae), commonly known as coconut palm. It is probably native to the Pacific islands, and today is cultivated almost throughout the tropics. It
the most cultivated and palm in the world, and currently is the main species producing vegetable fat. It is a plant that provides a wider range of products in the world, being a primary source of food, drink and shelter. The distribution of the coconut palm extends over most of the islands and the tropical coasts and in some places outside the tropics.
The main export is raw copra desiccated coconut followed.
diversity and potential of coconut contributes significantly to the economic sector in producing countries. 2.2 Background
coconut lethal yellowing (ALC) is considered as one of the most important diseases that affect the production of coconut, coconut trees has destroyed up to 100% in some countries (Harries 1978). It is caused by a phytoplasma, This devastating disease is found in Mexico, Honduras, Belize, Guatemala, USA (Florida), Jamaica, Cuba, Grand Cayman, Haiti and the Dominican Republic (Beakbane et al 1972).
Many of these countries have had disastrous experiences as in the case of Jamaica, where 100% of their plantations were destroyed in a period of four years, estimated losses in five million palms in a period of 20 years (Romney ( 1983). In Honduras has destroyed 90% of the coconut (Plavsic-Banjac et al., 1972).
is an organism that has characteristics similar to bacteria, have cytoplasm, ribosomes, DNA and is restricted to phloem cells. It is transmitted by insects is one of the most important Myndus crudus, Van Duzz (Homoptera: Cixidae) (Howard et al, 1983, Howard, 1995).
pathogen dispersal apparently occurs through two mechanisms, (McCoy et al 1983). One is through a central localized infection, ie, the disease appears in one or both palms and then randomly extends adjacent palms. The second mechanism of dispersion of the FTA is at large distances as discontinuous, followed by localized dispersal. The distance covered by these jumps can be tens of kilometers and are favored by high winds such as hurricanes. Therefore, the pathogen can spread very quickly as has happened in Mexico and Honduras, (Cordova, 1995).
M. crudus feed on diseased plants and then moved to healthy plants, thus spreading the disease. It is believed that the ornamental grass used to beautify the surroundings of the tourist areas is used for development (Piña, 1995). Have been reported in different strains of phytoplasma causing lethal yellowing of coconut in the Caribbean and Africa (Harrison et al.1994).
Symptoms of LAC in the Caribbean and Africa show similar, but differences were observed in the epidemiology and susceptibility in the materials, so it is assumed that different phytoplasmas cause disease. The coconut trees and palms likely die quickly, in a period between 4 and 6 months after the onset of symptoms (Eden-Green, 1995)
. ALC
symptoms observed in Puerto Plata, have been confirmed by the Scientific Research Centre of Yucatan (CICY) of Mexico, in collaboration with the University of Florida, United States, in samples submitted in 1997. These samples were analyzed by the technique of Chain Reaction Polymerase-PCR, they were positive to LAC. This technique has been. Used to detect the phytoplasma in the embryo of the fruit. (Cordova, et al, 2003).
This disease was reported for more than 40 years until 2006, being detected and confirmed a new outbreak in the south coast in the town of Boca Chica, (Martinez et al, 2007)
111. MATERIALS AND METHODS. 3.1 Location
The study will cover the North (Puerto Plata, Dajabón) and South (Andres Boca Chica) where the disease has been reported commercial plantations in the Northeast (Maria Trinidad Sanchez, Samana) and Eastern (Hato Mayor, El Seibo, La Altagracia, La Romana) of the Dominican Republic, where no disease has been reported. In order to confirm that the area remains free of ALC.
3.2 Type of research.
is a descriptive research to detect and characterize the genetic variability of phytoplasma LAC coconut palms in areas affected by CLA.
3.3. Selection of plants sampled.
In every place where the disease has been reported to be selected 5 plants showing symptoms typical of CLA and 5 asymptomatic plants. In each plant, take samples of leaves, stems and inflorescences to determine the presence or phytoplasma. In areas free of the disease, samples were taken from 10 plants.
receiver will use Global Positioning System (GPS) to mark the areas of advocacy, the points located were used to construct a map of disease distribution
3.4.
Sampling Samples were taken from the young leaves and inflorescence stem. For the sample was chosen with symptoms characteristic of the early stage of FTA. For the inflorescence, cut is closed before the open inflorescence stems and samples were taken by a hole 10 to 12 cm. deep, at a height of 1.5 meters de1.0 soil using an electric drill.
The collected samples will be placed on damp paper and deposited in a plastic bag previously identified. They are then placed in a cooler to be transported to the laboratory.
3.3 Analysis Laboratory
The samples will be taken to the laboratory of Virology of the Centre for Agricultural Technology (CENTA) of the Dominican Institute of Agriculture and Forestry Research (IDIAF). These are analyzed through Chain Reaction (PCR). The research
Three types of analysis:
a) nested PCR for detection of phytoplasmas.
b) Restriction fragments length polymerase (RFLPs) for the differentiation of genetic diversity.
c) Sequencing of the selected samples.
The DNA samples were amplified using universal primers P1-P7 for the presence of phytoplasma in the samples, then perform a nested PCR with specific primers and LY50F LysR. 3.4
phytoplasma detection
The PCR product will be fragmented to electrophoresis in agarose gel 5% to analyze the patterns of bands obtained. For detection of phytoplasma. The PCR product obtained will be sequenced to relate the similarity with other phytoplasmas in the regions area. Molecular characterization of races, we will make comparisons with different isolates of the pathogen to determine if there are different strains of phytoplasma in the Dominican Republic.
3.6 - Schedule of activities
Activity
EFMAMJJASONDEFMAM
Buy reactive
Sample Collection xxx xxx
Laboratory analysis results Analysis Presentation thesis
XX
Publication
3.5-budget for this research. ARTICLE
ITEM SPECIFICATIONS UNIT UNIT PRICE AMOUNT VALUE Value U.S. $ RD $
1 0.5 ul eppendorf tubes cover / 500 1 2000 2000 60.60 1.5
2 ul eppendorf tubes cover / 500 1 2500 2500 3 75.75
Punta micropipettes 1 -10 ul Cover / 1000 2700 1400 4 42.42
Case Punta ul micropipettes 200-1000 / 1000 2 1200 2400 5 72.72
Disposable Gloves Medium / Long Box / 100 3195585 17.72 6
Vials/50 started 24 bp 2 ul 2 7 7821.02 3910.51 237.00
Taq polymerase 250 units / ul 1 2 vials
280.00 4620 9240 8 10 mM dNTPs dATP, dCTP, dGTP and dTTP Kit 0.5 mL 1 160.00 5280 5280 9 PCR mix
September 1 4780 4780.00 144.80 liters liquid nitrogen
10 4,170,680 20.60
11 Grade Agarose PCR. Intermediate solution. Molecular weight resealable plastic bag < 1000 bp. Sobre /100 gr 1 7755 7755 235.00
12 6x10/10x 14 "boxes / 100 3,300,900 27.27
Binoculars 13 3500 3500 1 106.00
14 48841.02 1480.03 Sub-Total Operating Expenses
15 16 Fuel 12 viajes/35 gl / Km Travel 12 1770 2124 0 643.00
17 Per diem 2 technicians / Driver 36 300 1080 0 327.27
18 Salary / Labor Day 1 inflorescence sampling
8200 48.48 1600 19 6400.00 Thesis Preparation and printing 193.94
19 Sub-Total Expenditure 40040.00 1019.39
Op 20 Sub-Total Op Expenses / Reagents
88881.02 2499.42 8888.102 21 Contingencies 10%
249.90 Grand Total 97769.12 2749.36 22
Note: This is
The estimated budget for this inquiry, but have yet
the Agricultural Technology Centre IDIAF, laboratory equipment muestras.en analyzing the yucatan mexico is where conditions until the time for fulfillment.
Bibliography:
1 - Almeyda, Isidro, molecular 1998.Diagnóstico Coconut lethal yellowing,
Regional Research Center Southeast, Yucatan, Mexico.
2 - Beakbane, AB, Slater, CHW and Posnett, AF1972. Mycoplasmas in the phloem
of coconut, Cocos nucifera L, with lethal yellowing disease.
Journal of Horticulture Science.
3 - Export and Investment Center of the Dominican Republic (CEI-RD), 2005
4 - Cordova, I.1995.Estudiola intraplant distribution and dispersal of lethal yellowing in cocoteeros
using the chain Reccion polymerase.
5 - Eden-Green, SJ 1995. Brief history of yellowing research. In: C. Oropeza.,
WF Howard and GR. Absburner lethal yellowing research and practical aspects. pp. 17-33.
6- Harrison, N.A., Richardson, P.A., Kramer, J.B. and Tsai, J.H. 1994. Detection of
the Mycoplasmas-like organism associated with lethal yellowing disease of palm
in Florida by polymerase chain reaction Plant Pathology.
7- Harries, H.C.1978. Lethal yellowing disease of coconut in global perspective.
Philippines journal of coconut science. Florida, Estados Unidos
8- Howard, F.W., Norris, R.C. and Thomas, D.L. (1983). Evidence of transmission of palm lethal yellowing agents by a planthopper, Myndus crudus (Homptera: Cixiidae). Tropical Agriculture, Trinidad.
9- Howard, F.W. (1995). lethal vector yellowing study. Methods of experimental
transmission. In: C. Oropeza., WF Howard., And GR. Absburmer: lethal yellowing research
and Practical aspects.
10 - Hichez, E.1971. Lethal yellow in the Dominican Republic. Bulletin No.3
Plant Health Department, Secretary of State for Agriculture.
11 - Martinez, Desiré, 2006.Informe National Association of Hotels and
Restaurantes.Listin Journal, 19 marzo2006.
12-Ministry of Agriculture, 2006. Diagnosis of the Agricultural Sector, Assistant Secretary for Planning, Department of Agricultural Economics, Santo Domingo, Dominican Republic.
11 - Plavsic-Benjac, B., Hunt, P. and Maramorosch, K. (1972). Mycoplasma-like Bodies
associated with lethal yellowing disease of coconut palm. Phytopathology, 62: 298-2 - 12 - Romney, DH (1983). Brief review of coconut lethal yellowing. Indian coconut journal.
(This is a research work done by the Ing.Andrea
Felix)
. NOTICE
Introduction
coconut cultivation (Cocos nucifera L) is of economic, social and ecological to the Dominican Republic, which produces the currency, jobs and income, food and other products for local and international market. Besides coconut palms grace the beaches of the main tourist areas, providing its tropical beauty to the landscape, and therefore it which encourages tourists visit the country.
In Dominican Republic, the cultivated area is 22.491 ha. 359, 856 TAS. With an average annual production of 2,227, 872.8, units in the east and west coastal areas (Ministry of Agriculture, 2006). The production of fresh coconuts and products are intended for national and international markets, the latter generating U.S. $ 4, 553,143.00 Dollars (CEI-RD, 2005) by way of exports.
In 2006 the Dominican Republic was visited by 2, 612.256 tourists from different parts of the world, generating tourism input $ 3.750 and more than 300 thousand jobs (Martínez, 2006).
The coconut lethal yellowing disease (ALC) is considered the most devastating affects this crop, which has killed hundreds of thousands of palms in Key West, Florida, USA, from the Caribbean 1936.En has caused the disappearance of this crop, destroying millions of coconut palms in the last 40 years (OIRSA, 2002). CLA is caused by a phytoplasma, transmitted by the insect vector crudus Myndus Van Duzee, of the order Homoptera: Family Cixiidae. (Howard, 1983).
The ALC has been reported in the Dominican Republic several times since 1962, affecting domestic plantations in locations the province of Puerto Plata, Santiago and Dajabón (Hichez, 1971). Recently it was reported an outbreak in the town of Andrés, Boca Chica, Santo Domingo Province. (Martinez et al, 2007).
The detection of this disease is carried by the technique of reaction polymerase chain (PCR), which is a molecular technique that amplifies nucleic acid bands.
Because these pathogens can not grow invitro, recommended diagnostic technique using Chain Reaction (PCR), which is a molecular technique that amplifies nucleic acid bands. The PCR is recommended for detection of phytoplasmas and genetic characterization of these. Phytoplasma known races allow management to devise strategies to prevent the spread of disease and damage in commercial plantations.
I.
PROBLEM 1.1. Description and formulation of the problem.
The coconut lethal yellowing disease (ALC) has been reported in the Dominican Republic since 1962, affecting domestic plantations in the villages of the provinces of Puerto Plata, Santiago and Dajabón. In each occurrence of the disease have been implemented measures such as slashing and burning of diseased plants to prevent the rapid dispersion the same (Hichez, 1971).
The most recent occurrence of CLA was reported in 2006 in the east coast in the town of Andrés, Boca Chica, with a larger number of symptomatic plants, which reported outbreaks in previous years in the North, (Martinez, personal communication 2006).
This disease represents a potential threat to the production of coconuts in the Dominican Republic, as this has been reported as the most devastating crop of coconut palms; Initial symptoms are characterized by the premature fall of the fruits of all sizes, the lower leaves, intermediate and higher turn yellow, the heads and knives become necrotic, in the final stage all the leaves fall, leaving only the stem erect (Córdova, 1995). 1.2 OBJECTIVES
objective 1.2.1:
Diagnosis and characterization of strains of phytoplasmas coconut lethal yellowing.
1.2.2 Specific objectives
a) determine the presence of phytoplasma.
b) Genetic differentiation of phytoplasma strains in the country.
c) Establish the relationship of the phytoplasmas found in the Dominican Republic with other países.México, Honduras, Belize, Guatemala, USA (Florida), Jamaica, Cuba, Cayman and Haiti.
1.3 - Justification
For the Dominican Republic coconut cultivation is of great importance because it is used in agriculture and tourism, generating foreign exchange for the country as part of the export of the product and concept as tourism, attracted by the coconut.
The production of fresh coconuts, grated, dried, and products for the cosmetics industry, food and medicine is intended for local and international markets, both the United States, European Union, Canada, West Indies Children, Virgin Islands, Brazil, Puerto Rico, Haiti and others. Foreign exchange earnings in 2005, this concept was worth $ 2, 282, 987.80, USD. In 2004 and $ 4, 553,143.00, USD (CEI-RD, 2005). It is important to note that Nationwide thousands of families depend on this crop for their livelihood.
coconut palms beaches located in major tourist areas, provide the tropical beauty of the landscape, which encourages tourists visit. In 2006 the Dominican Republic was visited by 2,612,256 tourists from different parts of the world, generating tourism $ 3.750 input. More than 300 thousand. Jobs (Martínez, 2006).
business opportunities for The Dominican Republic is developing exports, as the nations of North America, Europe, South America and the Caribbean require greater amount of fresh coconut, processed and a wide variety of products. Today has resumed the use of coconut oil for culinary purposes, such as virgin coconut oil. In the United States and Europe, ½ liter sells for $ 16.00 and U.S. $ 15.75, respectively. This represents an opportunity to diversify production and increase foreign exchange earnings from this crop (CEI-RD 2006). 1:4
Diagnosis and Characterization
The diagnosis and understanding of the genetic diversity of the pathogen in the country help design management strategies to prevent the spread of disease and damage to areas of commercial production. So the knowledge of the races will help design management strategies to prevent the spread and damage to commercial plantations.
This research would provide the basis for the continuity of production and the benefits it provides to more than 1500 coconut farmers, processors, exporters and other members of the Dominican Republic in the production chain as well as foreign exchange earnings and employment sources both sectors and the preservation of the natural landscape that give the coconut palms to the country's beaches. Creating the basis for both sectors are more competitive and sustainable, especially in times of opening new markets.
1.4 Scope and limitations
1.4.1 Scope The study will include the towns of the North (Puerto Plata, Santiago, Dajabón) and Southeast (Andrés, Boca Chica) where the disease has been reported and in the production regions Northeast Commercial (Maria Trinidad Sanchez, Samana) and Eastern (Hato Mayor, El Seibo, La Altagracia, La Romana) in the country. Which has not been reported. and to ensure that these areas are free of the disease. 1.4.2 Limitations
To determine the sequence of base pairs of possible phytoplasma in the country, will be sent to international centers engaged in such activity, as in the Dominican Republic there are no possibilities for these techniques.
11. THEORETICAL FRAMEWORK 2.1 General
Cocos nucifera L.
(Belonging to the family Arecaceae), commonly known as coconut palm. It is probably native to the Pacific islands, and today is cultivated almost throughout the tropics. It
the most cultivated and palm in the world, and currently is the main species producing vegetable fat. It is a plant that provides a wider range of products in the world, being a primary source of food, drink and shelter. The distribution of the coconut palm extends over most of the islands and the tropical coasts and in some places outside the tropics.
The main export is raw copra desiccated coconut followed.
diversity and potential of coconut contributes significantly to the economic sector in producing countries. 2.2 Background
coconut lethal yellowing (ALC) is considered as one of the most important diseases that affect the production of coconut, coconut trees has destroyed up to 100% in some countries (Harries 1978). It is caused by a phytoplasma, This devastating disease is found in Mexico, Honduras, Belize, Guatemala, USA (Florida), Jamaica, Cuba, Grand Cayman, Haiti and the Dominican Republic (Beakbane et al 1972).
Many of these countries have had disastrous experiences as in the case of Jamaica, where 100% of their plantations were destroyed in a period of four years, estimated losses in five million palms in a period of 20 years (Romney ( 1983). In Honduras has destroyed 90% of the coconut (Plavsic-Banjac et al., 1972).
is an organism that has characteristics similar to bacteria, have cytoplasm, ribosomes, DNA and is restricted to phloem cells. It is transmitted by insects is one of the most important Myndus crudus, Van Duzz (Homoptera: Cixidae) (Howard et al, 1983, Howard, 1995).
pathogen dispersal apparently occurs through two mechanisms, (McCoy et al 1983). One is through a central localized infection, ie, the disease appears in one or both palms and then randomly extends adjacent palms. The second mechanism of dispersion of the FTA is at large distances as discontinuous, followed by localized dispersal. The distance covered by these jumps can be tens of kilometers and are favored by high winds such as hurricanes. Therefore, the pathogen can spread very quickly as has happened in Mexico and Honduras, (Cordova, 1995).
M. crudus feed on diseased plants and then moved to healthy plants, thus spreading the disease. It is believed that the ornamental grass used to beautify the surroundings of the tourist areas is used for development (Piña, 1995). Have been reported in different strains of phytoplasma causing lethal yellowing of coconut in the Caribbean and Africa (Harrison et al.1994).
Symptoms of LAC in the Caribbean and Africa show similar, but differences were observed in the epidemiology and susceptibility in the materials, so it is assumed that different phytoplasmas cause disease. The coconut trees and palms likely die quickly, in a period between 4 and 6 months after the onset of symptoms (Eden-Green, 1995)
. ALC
symptoms observed in Puerto Plata, have been confirmed by the Scientific Research Centre of Yucatan (CICY) of Mexico, in collaboration with the University of Florida, United States, in samples submitted in 1997. These samples were analyzed by the technique of Chain Reaction Polymerase-PCR, they were positive to LAC. This technique has been. Used to detect the phytoplasma in the embryo of the fruit. (Cordova, et al, 2003).
This disease was reported for more than 40 years until 2006, being detected and confirmed a new outbreak in the south coast in the town of Boca Chica, (Martinez et al, 2007)
111. MATERIALS AND METHODS. 3.1 Location
The study will cover the North (Puerto Plata, Dajabón) and South (Andres Boca Chica) where the disease has been reported commercial plantations in the Northeast (Maria Trinidad Sanchez, Samana) and Eastern (Hato Mayor, El Seibo, La Altagracia, La Romana) of the Dominican Republic, where no disease has been reported. In order to confirm that the area remains free of ALC.
3.2 Type of research.
is a descriptive research to detect and characterize the genetic variability of phytoplasma LAC coconut palms in areas affected by CLA.
3.3. Selection of plants sampled.
In every place where the disease has been reported to be selected 5 plants showing symptoms typical of CLA and 5 asymptomatic plants. In each plant, take samples of leaves, stems and inflorescences to determine the presence or phytoplasma. In areas free of the disease, samples were taken from 10 plants.
receiver will use Global Positioning System (GPS) to mark the areas of advocacy, the points located were used to construct a map of disease distribution
3.4.
Sampling Samples were taken from the young leaves and inflorescence stem. For the sample was chosen with symptoms characteristic of the early stage of FTA. For the inflorescence, cut is closed before the open inflorescence stems and samples were taken by a hole 10 to 12 cm. deep, at a height of 1.5 meters de1.0 soil using an electric drill.
The collected samples will be placed on damp paper and deposited in a plastic bag previously identified. They are then placed in a cooler to be transported to the laboratory.
3.3 Analysis Laboratory
The samples will be taken to the laboratory of Virology of the Centre for Agricultural Technology (CENTA) of the Dominican Institute of Agriculture and Forestry Research (IDIAF). These are analyzed through Chain Reaction (PCR). The research
Three types of analysis:
a) nested PCR for detection of phytoplasmas.
b) Restriction fragments length polymerase (RFLPs) for the differentiation of genetic diversity.
c) Sequencing of the selected samples.
The DNA samples were amplified using universal primers P1-P7 for the presence of phytoplasma in the samples, then perform a nested PCR with specific primers and LY50F LysR. 3.4
phytoplasma detection
The PCR product will be fragmented to electrophoresis in agarose gel 5% to analyze the patterns of bands obtained. For detection of phytoplasma. The PCR product obtained will be sequenced to relate the similarity with other phytoplasmas in the regions area. Molecular characterization of races, we will make comparisons with different isolates of the pathogen to determine if there are different strains of phytoplasma in the Dominican Republic.
3.6 - Schedule of activities
Activity
EFMAMJJASONDEFMAM
Buy reactive
Sample Collection xxx xxx
Laboratory analysis results Analysis Presentation thesis
XX
Publication
3.5-budget for this research. ARTICLE
ITEM SPECIFICATIONS UNIT UNIT PRICE AMOUNT VALUE Value U.S. $ RD $
1 0.5 ul eppendorf tubes cover / 500 1 2000 2000 60.60 1.5
2 ul eppendorf tubes cover / 500 1 2500 2500 3 75.75
Punta micropipettes 1 -10 ul Cover / 1000 2700 1400 4 42.42
Case Punta ul micropipettes 200-1000 / 1000 2 1200 2400 5 72.72
Disposable Gloves Medium / Long Box / 100 3195585 17.72 6
Vials/50 started 24 bp 2 ul 2 7 7821.02 3910.51 237.00
Taq polymerase 250 units / ul 1 2 vials
280.00 4620 9240 8 10 mM dNTPs dATP, dCTP, dGTP and dTTP Kit 0.5 mL 1 160.00 5280 5280 9 PCR mix
September 1 4780 4780.00 144.80 liters liquid nitrogen
10 4,170,680 20.60
11 Grade Agarose PCR. Intermediate solution. Molecular weight resealable plastic bag < 1000 bp. Sobre /100 gr 1 7755 7755 235.00
12 6x10/10x 14 "boxes / 100 3,300,900 27.27
Binoculars 13 3500 3500 1 106.00
14 48841.02 1480.03 Sub-Total Operating Expenses
15 16 Fuel 12 viajes/35 gl / Km Travel 12 1770 2124 0 643.00
17 Per diem 2 technicians / Driver 36 300 1080 0 327.27
18 Salary / Labor Day 1 inflorescence sampling
8200 48.48 1600 19 6400.00 Thesis Preparation and printing 193.94
19 Sub-Total Expenditure 40040.00 1019.39
Op 20 Sub-Total Op Expenses / Reagents
88881.02 2499.42 8888.102 21 Contingencies 10%
249.90 Grand Total 97769.12 2749.36 22
Note: This is
The estimated budget for this inquiry, but have yet
the Agricultural Technology Centre IDIAF, laboratory equipment muestras.en analyzing the yucatan mexico is where conditions until the time for fulfillment.
Bibliography:
1 - Almeyda, Isidro, molecular 1998.Diagnóstico Coconut lethal yellowing,
Regional Research Center Southeast, Yucatan, Mexico.
2 - Beakbane, AB, Slater, CHW and Posnett, AF1972. Mycoplasmas in the phloem
of coconut, Cocos nucifera L, with lethal yellowing disease.
Journal of Horticulture Science.
3 - Export and Investment Center of the Dominican Republic (CEI-RD), 2005
4 - Cordova, I.1995.Estudiola intraplant distribution and dispersal of lethal yellowing in cocoteeros
using the chain Reccion polymerase.
5 - Eden-Green, SJ 1995. Brief history of yellowing research. In: C. Oropeza.,
WF Howard and GR. Absburner lethal yellowing research and practical aspects. pp. 17-33.
6- Harrison, N.A., Richardson, P.A., Kramer, J.B. and Tsai, J.H. 1994. Detection of
the Mycoplasmas-like organism associated with lethal yellowing disease of palm
in Florida by polymerase chain reaction Plant Pathology.
7- Harries, H.C.1978. Lethal yellowing disease of coconut in global perspective.
Philippines journal of coconut science. Florida, Estados Unidos
8- Howard, F.W., Norris, R.C. and Thomas, D.L. (1983). Evidence of transmission of palm lethal yellowing agents by a planthopper, Myndus crudus (Homptera: Cixiidae). Tropical Agriculture, Trinidad.
9- Howard, F.W. (1995). lethal vector yellowing study. Methods of experimental
transmission. In: C. Oropeza., WF Howard., And GR. Absburmer: lethal yellowing research
and Practical aspects.
10 - Hichez, E.1971. Lethal yellow in the Dominican Republic. Bulletin No.3
Plant Health Department, Secretary of State for Agriculture.
11 - Martinez, Desiré, 2006.Informe National Association of Hotels and
Restaurantes.Listin Journal, 19 marzo2006.
12-Ministry of Agriculture, 2006. Diagnosis of the Agricultural Sector, Assistant Secretary for Planning, Department of Agricultural Economics, Santo Domingo, Dominican Republic.
11 - Plavsic-Benjac, B., Hunt, P. and Maramorosch, K. (1972). Mycoplasma-like Bodies
associated with lethal yellowing disease of coconut palm. Phytopathology, 62: 298-2 - 12 - Romney, DH (1983). Brief review of coconut lethal yellowing. Indian coconut journal.
(This is a research work done by the Ing.Andrea
Felix)
. NOTICE
How Many Calories Are In Stir Fry Noodles
SIGATOKA BLACK CULTURE
SEVERITY ASSESSMENT BIOLOGICAL PRODUCTION IN PLANTATION RD
INTRODUCTION The development and combat Black Sigatoka in banana and plantain, are two items very closely.
The first is the characteristic vegetative plant present a constant rate of leaf emergence, which leads to permanently this exposing new tissue to possible pathogen inoculation.
The other factor relates to the conditions of the regions most banana and plantain in Latin America, representing feature very favorable temperature and humidity on the biology of the fungus. An exception is high altitude where it is cultivated banana, so particular, in association with coffee or in some regions of marked dry season but where irrigation is practiced, which tend to create certain conditions that may be favorable to biology the fungus.
These characteristics, together with the action of the fungicide products have caused some assessment strategies to combat Black Sigatoka, within which the warning system has shown a high efficiency, reduced costs and less environmental pollution .
The methodology of the notice to combat this disease, based on the analysis of biological and climatic
descriptors so that fungicide applications can be made only when
initial development of the infection and when the parameters Weather you are favorable to the fungus.
This facilitates timely intervention is, by reversing the attack, a wider spacing between courses of treatment, lower levels of disease and consequently healthier plantations better articulate their productive potential.
as notice to combat Black Sigatoka refers to two systems: the notice biological and bioclimatic notice. This time we will only biological notice as to the bioclimatic notice requires certain conditions, which we have not yet. NOTICE
BIOLOGICAL
This methodology is independent of bioclimatic notice. In areas
which initiates the development of notice is the first step and not until years later that can be combined with climate studies to establish the bioclimatic notice.
biological warning system is based on the analysis of biological descriptors (disease assessment in relation to the plant, which can be concluded in the Statement of Changes).
For this purpose, conduct weekly evaluations so that the onset of disease symptoms on young leaves (II, III and IV) enable a constant and "accounting" the development of the disease.
Instruments This basic system is the observation plot and the curve of the state of evolution, which reflects the results of field observations.
OBSERVATION SITE
order to "measure" the constant development of the disease, it is necessary to weekly observations on the same plant until its height allow comments on the sheets II, III and IV or up to the issuance of the inflorescence (calving).
There are no rules in terms of density plots needed in a given area. This depends on the homogeneity of environmental conditions on the banana plantations under the premise that different farms (particularly in areas with small farms) have a similar operation.
To establish the place of observation in adult plantations should be planted in lots of 40 plants of which only 10 will be assessed. Avoid
plots near the location of facilities such as houses, packing plants, power lines, roads, etc.., Or where there are obstacles to flight of the aircraft that can not be eliminated.
farms with new plantings, the 10 plants evaluated can be placed with a wider distribution, it should be borne in mind that these measures should be easily located by the manager of the readings. Whether
plots established or selected plants in new plantings, they must be individually identified, it is recommended to use alpha-numeric. In these signs the letter corresponding to the line or row of plants and the number to the position of the plant in the row.
For ease of location of plants to observe, must be labeled at the beginning of the row with the letter that corresponds and plants, in addition to placing an identification card with the alpha-numeric, it is painted on the pseudostem, a ring that may be visible from a distance.
When you can not plant new plots (40 plants) for the observation plots, proceed to choose the children with 10 developed leaves of a plot in production and to establish with these the observation plot.
REPLACEMENT SITE
Due to the urgent need for consistent information should be provided for the availability of plants in which evaluations should be continued once reaching the issue of inflorescence which are being evaluated.
To this end, the plots should be established early enough replacement for that time. This anticipation depends on the material being employed, depending on whether rhizomes or seedlings from nurseries (tissue culture or shoots), the time to be determined and weather conditions.
Generally the time required to set the replacement parcel is approximately two (2) months before being tested which reaches parity.
is important to note that reading the time to abandon a plot should coincide with the second reading at the place of replacement, which means that in a given week, will be read out on the plots but in the manner indicated because otherwise, there may be gaps in information and problems with the results obtained.
In banana-producing areas that have a prolonged dry period and there is no irrigation, should be avoided
establish plots for the same. In these cases,
same shall be set at the start of the rainy season. Pose a problem
plots replacement? In fact the only drawback to this work, is the reluctance of producers to slaughter plants should lead to replacement plots. To this end it is necessary that it has a clear awareness of the
benefits derived from implementation of the application of this system as sacrificing a few plants, will be largely offset by the economy spraying cycles in the whole house or a given area and also it will provide a better health condition of the plantation and all the additional benefits to be derived from it.
There is an option to replacement plot establishment, which is to continue the observations on children.
This should be taken into account considerations such as:
• The system could run on low-bearing cultivars such as the Great Dwarf, since most other high as Valery, children sores to be very high before develop their functional leaves.
• The behavior of the state of evolution children differs from that seen in the templates, although the trend is similar. The amplitude of the curve representing the evaluation of the disease differs in both cases, which could cause difficulties of interpretation.
• If you decide to work with children, should be carried out simultaneously by a given time period, the respective assessments in both types of material so that the staff responsible for the interpretation of results and recommendations in each materials about the disease itself.
EVALUATION METHODOLOGY
The "Statement of Changes" is the expression of disease development in terms of development of plant material. It is therefore necessary when carrying out evaluations, taking into account the growth of the plant (leaf emission) as well as symptoms (stages) of the disease.
To start data collection, when first reading the plants must have a minimum of eight (8) leaves, preferably 10, and shall correspond to 10 plants with normal growth and uniform, properly distributed within the plot observation.
In this first reading, the total number of leaves present in the plant, is marked at the base of the midrib of the leaf 1, which is that wide open after cigarette or candle.
Weekly, sheet 1 will be marked with the progressive number that corresponds to the total leaf emergence at that time (10, 11.12, etc).
This information is recorded for each respective plant in the form of the EFA column field data (see example). In addition to the total number of leaves, must be made on state of development of cigarette or candle, which is noted on the form as a decimal fraction of the total issued to the observation sheets.
This decimal fraction will correspond to each stage of development of the cigarette or candle, it is noted in Figure 1.
-Figure
cigar-
states
OBSERVATION OF DISEASE AND CALCULATION OF THE STATE OF EVOLUTION.
As noted above, on a weekly basis should be evaluated the same plants and at every opportunity, always leaves II, III, and IV.
The rationale behind this rule is that:
• New leaves are usually first to become infected.
• The methodology is to intervene in the disease in its earliest stages of development.
• The symptoms on a same sheet may eventually be observed by more than a week and with it, as they have evolved.
To determine the presence of different stages, observation or search starts on the underside of semilimbo apical left hand as this being the first part of the sheet is exposed to unroll a cigarette or candle, is the area first to be inoculated with the disease.
From this area you look at the rest of the blade for the most advanced stage of disease as well as an estimate of the number of symptoms present (see stages). Stage
Black Sigatoka
This information is what takes the form for purposes of such calculations, scoring in the columns for each leaf (range Leaf). For each one is scored only one state, the highest and added a qualification that will be + (plus) when their density is estimated that more than 50 symptoms or-(less) when less than 50.
in banana plantations under chemical control and for this methodology is very difficult to find stages over 2 -. Only in very critical cases, probably influenced by hot spots, you will find cases of 2 +.
Symptoms usually controlled should not be taken into account during data collection, however, it is sometimes difficult to differentiate these symptoms of living. In case of uncertainty, it is preferable not to consider it and note the symptoms they are.
Although not part of the methodology or the calculations to determine the state of evolution, can also be observed and recorded, the information on the youngest leaf sick (HMJE) and the youngest leaf necrotic (HMSN), which gives a quick idea of \u200b\u200bthe condition of the plantation over Black.
From these observations, the calculations proceed as follows.
In the first evaluation, because they can not obtain the data rate of leaf emergence, as no one has the last leaf emission data (EFP), was not able to get the State Assessment data. What we have achieved this opportunity is the gross amount (SB).
The second data collection, as well as the following, you can calculate the rhythm of leaf (REF) for each plant, adding and subtracting from the current week's data (EFA-Emission Current Leaf), the data leaf emission obtained for the same plant, the previous week (EFP = Past Issue Leaf).
EFA-VET REF =
Adding the REF for each plant, and dividing this sum (S REF) by the number of days (N) that elapsed between the two observations, the data obtained Emission Rate Foliar Present (REFI) for the batch of plants under observation. REFI = SREF
N
then the information must be generated called Candela Correction (CC). This is obtained by multiplying in each plant, the value assigned to its cigarette or candle
by the number of leaves (II, III and IV) found with symptoms of the disease.
For example, if the plant A5, candela state corresponded to 4, the 3-leaf disease were observed, then CC = 4x3 = 12.
From CC data, we obtain the coefficient of Evolution (CE). This is achieved by multiplying the sum of CC for all plants by 2, which is a preset factor.
CE = S CC x 2
To finish with the necessary information related to the development of the plant (leaf development), is just get the data of Foliar Weighted Emission Rate (REFp), which is only the promise of Rate the amount of leaf present.
REFp = REF + REFI 2
The information in plant development (leaf emergence) is required to combine with the factors of clinical expressed in the plant, leading to obtain the Statement of Changes (EE) of the disease and led to a graph representing the behavior going development of the disease in the plantation.
This other process starts with the calculation of the gross amount (SB), for it is necessary to add the same amount of leaf numbering in all 10 plants tested showed the same stage (columns in the form number of damaged leaves), 5 with stage III leaves 1 +, for example.
The sum of the product of the multiplication of these data, the severity coefficients, given below for each sheet and each stage results in the gross amount (SB).
Table 2. Severity
coefficients for calculating the gross amount. Leaf Stadium
II III IV 1 to 60 40 20
1 + 80 60 40
2-100 80 60
2 + 120 100 80
3 - 140 120 100
3 + 160 140 120
As an example, in a given plantation is located in the observation plot is the stage 1 - sheet II in two plants, two sheet plants III and IV leaf of two plants.
Stage 1 + is III found in the leaf of a plant and leaf of two plants IV. Stage 2 - is in the leaf III on two floors while stage 2 + is in the leaf IV 3 floors, information that is configured and calculated in Table 3.
Table 3.
Number of Leaves Leaves Damaged Damaged coefficient
Stadiums
II III IV II III IV
1 to 2 February 2120 80 40
1 + 1 2 60 80
2 - 2 160 2 + 3 130_______
To continue shall calculate the sum of Evolution, which is obtained by subtracting the coefficient of Evolution (and as calculated above) of the gross amount.
SEV = SB - CE
This data (SEV) is multiplied by the Weighted Average Rate of leaf emergence and results in the State of Evolution (EE) of the disease for that week.
EE = SEV x REFI
This figure, taken to a graph is by allowing the trend or slope of the same between two or more weeks, to define when you make an application or fungicide treatment.
Interpretation of Results
The most important factor that can decide when to apply, not the amount of infection found in plants, but the evolution of the disease, which is reflected in the curve of a graph.
There is, in principle, a critical threshold. Only experience levels may be setting some special consideration to be defined for different sectors.
is logical that a lower level to maintain the state of development, better health will be planting and, consequently, fewer applications and fungicide treatments. This is achieved only by the applications in the most timely manner possible.
ASSESSMENT OF THE STATE OF INFECTION
You must have a clear and accurate picture of the health status of the property, to prevent severe damage to the crop and its production. For this reason we must make periodic evaluations (every week or two) on the incidence and severity of Black Sigatoka on each farm.
Below is the modified Stover methodology for the evaluation of incidence and severity of the disease, which will allow us to be properly built on the plant health of planting.
METHODOLOGY OF PROPERTY BY STOVER Gauhl
The incidence and severity assessment using the modified Stover methodology, yields fairly detailed information on the health status of the plantation.
Figure 1 shows the six degrees that includes the scale changed Gauhl Stover (1989).
The system consists of a visual estimation of leaf area affected in all the leaves around flowering plants, without having to download the sheet.
This assessment takes into account all the leaves except the leaf present fire, and leaves overwhelmed. The nearest leaf to leaf blade is considered candela No.1
FIGURE 1. Scale changed Gauhl Stover (1989).
. Counting is easy considering the spiral distribution (odd and even) from right to left from sections 1 and 2 (Figure 2), counting down. To determine the leaf area affected must visually estimated the total area covered by all the symptoms of the disease in each sheet and calculate the percentage of leaf covered by the symptoms. For this it is necessary to have a pattern that divides the sheet into percentage ratios, as shown in Figure 1.
The following is an example of the evaluation sheet (Table 1), as well as calculations to be performed to obtain the variables generated by this method.
TABLE 1. Example of calculating the number of leaves per plant (H / P).
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P
1 0 0 0 0 0 0 0 0 1 1 2 2 12 2 0 0
0 0 0 0 0 0 0 0 1 2 12
3 0 0 0 0 0 0 0 0 0 0 0 0 2 13
4 0 0 0 0 0 0 0 0 0 0 0 1 1 2 14
5 0 0 0 0 0 0 0 0 0 1 1 2 2 13 total 64
averaged 12.8
The number in each box indicates the degree of infection that has each page based on a scale of 0 a6 according to Figure 1.
To obtain the number of leaves per plant, all leaves counted and divided by the number of plants tested (Table 1). The number of leaf per plant was extracted from the last sheet is indicated (colored) in the formula assessment.
youngest diseased leaf (HMJE) is also on average younger leaves (colored) showing visible symptoms of the disease. (Table 2).
The HMJE gives an indication of disease progression. In other words, the younger the leaf with more symptoms, the greater the incidence of the disease, and arguably also the severity.
TABLE 2. Calculation of the youngest leaf sick (HMJE).
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P HMJE
1 0 0 0 0 0 0 0 0 1 1 2 2 9 2 0 0 0
0 0 0 0 0 0 0 1 2 11
3 0 0 0 0 0 0 0 0 0 0 0 0 2 13
4 0 0 0 0 0 0 0 0 0 0 0 1 1 2 12
5 0 0 0 0 0 0 0 0 0 1 1 2 2 10 total 55
average 11
Finally, to obtain the percentage of leaves infected by grade, the number of leaves in each grade, divided by the total number leaves and multiplied by 100 (Table 3).
The total percentage of infected leaves were obtained by adding the value of all grades, from first to sixth.
TABLE 3. Calculation of the percentage of infected leaves (% HJ)
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P HMJE 0 1 2 3 4 5 6
1 0 0 0 0 0 0 0 0 1 1 2 2 8 2 2
2 0 0 0 0 0 0 0 0 0 0 1 2 10 1 1
3 0 0 0 0 0 0 0 0 0 0 0 0 2 12 0 1
4 0 0 0 0 0 0 0 0 0 0 0 1 February 1, 1911 February 1
5 0 0 0 0 0 0 0 0 0 1 1 2 2 9 2 2 Total 50 7 July
averaged 78 November 1911
EXAMPLE: Percentage of leaves with grade 1: Floor 1
: 2 leaves
Floor 2: 1 sheet
Floor 3: 0 sheets floor
4: 2 sheets
Plant 5: 2 TOTAL 7
leaves leaves with grade 1
evaluated TOTAL SHEETS: 64 SHEETS WITH GRADE
% 1 = 7 x 100 = 11.0
64 TOTAL = SUM% leaves infected leaves in each grade (from 1 to 6) = 11 + 11 + 0 + 0 + 0 + 0 = 22
However, the total percentage of infected leaves underestimates the severity of the disease and that is why the use of a weighted average of infection (PPI) has been suggested to obtain a more accurate value. Its calculation is obtained by multiplying the percentage of leaves in each grade by the corresponding value of the magnitude of modified Stover. Each result is added and the total is divided by 100.
EXAMPLE: Weighted Average of infection (PPI)
SUM (% x blades in each respective grade level)
PPI = 100 (11 x 1) + (11 x 2) +...... .. + (0 x 6) PPI = 100
PPI = 0.33
INTRODUCTION The development and combat Black Sigatoka in banana and plantain, are two items very closely.
The first is the characteristic vegetative plant present a constant rate of leaf emergence, which leads to permanently this exposing new tissue to possible pathogen inoculation.
The other factor relates to the conditions of the regions most banana and plantain in Latin America, representing feature very favorable temperature and humidity on the biology of the fungus. An exception is high altitude where it is cultivated banana, so particular, in association with coffee or in some regions of marked dry season but where irrigation is practiced, which tend to create certain conditions that may be favorable to biology the fungus.
These characteristics, together with the action of the fungicide products have caused some assessment strategies to combat Black Sigatoka, within which the warning system has shown a high efficiency, reduced costs and less environmental pollution .
The methodology of the notice to combat this disease, based on the analysis of biological and climatic
descriptors so that fungicide applications can be made only when
initial development of the infection and when the parameters Weather you are favorable to the fungus.
This facilitates timely intervention is, by reversing the attack, a wider spacing between courses of treatment, lower levels of disease and consequently healthier plantations better articulate their productive potential.
as notice to combat Black Sigatoka refers to two systems: the notice biological and bioclimatic notice. This time we will only biological notice as to the bioclimatic notice requires certain conditions, which we have not yet. NOTICE
BIOLOGICAL
This methodology is independent of bioclimatic notice. In areas
which initiates the development of notice is the first step and not until years later that can be combined with climate studies to establish the bioclimatic notice.
biological warning system is based on the analysis of biological descriptors (disease assessment in relation to the plant, which can be concluded in the Statement of Changes).
For this purpose, conduct weekly evaluations so that the onset of disease symptoms on young leaves (II, III and IV) enable a constant and "accounting" the development of the disease.
Instruments This basic system is the observation plot and the curve of the state of evolution, which reflects the results of field observations.
OBSERVATION SITE
order to "measure" the constant development of the disease, it is necessary to weekly observations on the same plant until its height allow comments on the sheets II, III and IV or up to the issuance of the inflorescence (calving).
There are no rules in terms of density plots needed in a given area. This depends on the homogeneity of environmental conditions on the banana plantations under the premise that different farms (particularly in areas with small farms) have a similar operation.
To establish the place of observation in adult plantations should be planted in lots of 40 plants of which only 10 will be assessed. Avoid
plots near the location of facilities such as houses, packing plants, power lines, roads, etc.., Or where there are obstacles to flight of the aircraft that can not be eliminated.
farms with new plantings, the 10 plants evaluated can be placed with a wider distribution, it should be borne in mind that these measures should be easily located by the manager of the readings. Whether
plots established or selected plants in new plantings, they must be individually identified, it is recommended to use alpha-numeric. In these signs the letter corresponding to the line or row of plants and the number to the position of the plant in the row.
For ease of location of plants to observe, must be labeled at the beginning of the row with the letter that corresponds and plants, in addition to placing an identification card with the alpha-numeric, it is painted on the pseudostem, a ring that may be visible from a distance.
When you can not plant new plots (40 plants) for the observation plots, proceed to choose the children with 10 developed leaves of a plot in production and to establish with these the observation plot.
REPLACEMENT SITE
Due to the urgent need for consistent information should be provided for the availability of plants in which evaluations should be continued once reaching the issue of inflorescence which are being evaluated.
To this end, the plots should be established early enough replacement for that time. This anticipation depends on the material being employed, depending on whether rhizomes or seedlings from nurseries (tissue culture or shoots), the time to be determined and weather conditions.
Generally the time required to set the replacement parcel is approximately two (2) months before being tested which reaches parity.
is important to note that reading the time to abandon a plot should coincide with the second reading at the place of replacement, which means that in a given week, will be read out on the plots but in the manner indicated because otherwise, there may be gaps in information and problems with the results obtained.
In banana-producing areas that have a prolonged dry period and there is no irrigation, should be avoided
establish plots for the same. In these cases,
same shall be set at the start of the rainy season. Pose a problem
plots replacement? In fact the only drawback to this work, is the reluctance of producers to slaughter plants should lead to replacement plots. To this end it is necessary that it has a clear awareness of the
benefits derived from implementation of the application of this system as sacrificing a few plants, will be largely offset by the economy spraying cycles in the whole house or a given area and also it will provide a better health condition of the plantation and all the additional benefits to be derived from it.
There is an option to replacement plot establishment, which is to continue the observations on children.
This should be taken into account considerations such as:
• The system could run on low-bearing cultivars such as the Great Dwarf, since most other high as Valery, children sores to be very high before develop their functional leaves.
• The behavior of the state of evolution children differs from that seen in the templates, although the trend is similar. The amplitude of the curve representing the evaluation of the disease differs in both cases, which could cause difficulties of interpretation.
• If you decide to work with children, should be carried out simultaneously by a given time period, the respective assessments in both types of material so that the staff responsible for the interpretation of results and recommendations in each materials about the disease itself.
EVALUATION METHODOLOGY
The "Statement of Changes" is the expression of disease development in terms of development of plant material. It is therefore necessary when carrying out evaluations, taking into account the growth of the plant (leaf emission) as well as symptoms (stages) of the disease.
To start data collection, when first reading the plants must have a minimum of eight (8) leaves, preferably 10, and shall correspond to 10 plants with normal growth and uniform, properly distributed within the plot observation.
In this first reading, the total number of leaves present in the plant, is marked at the base of the midrib of the leaf 1, which is that wide open after cigarette or candle.
Weekly, sheet 1 will be marked with the progressive number that corresponds to the total leaf emergence at that time (10, 11.12, etc).
This information is recorded for each respective plant in the form of the EFA column field data (see example). In addition to the total number of leaves, must be made on state of development of cigarette or candle, which is noted on the form as a decimal fraction of the total issued to the observation sheets.
This decimal fraction will correspond to each stage of development of the cigarette or candle, it is noted in Figure 1.
-Figure
cigar-
states
OBSERVATION OF DISEASE AND CALCULATION OF THE STATE OF EVOLUTION.
As noted above, on a weekly basis should be evaluated the same plants and at every opportunity, always leaves II, III, and IV.
The rationale behind this rule is that:
• New leaves are usually first to become infected.
• The methodology is to intervene in the disease in its earliest stages of development.
• The symptoms on a same sheet may eventually be observed by more than a week and with it, as they have evolved.
To determine the presence of different stages, observation or search starts on the underside of semilimbo apical left hand as this being the first part of the sheet is exposed to unroll a cigarette or candle, is the area first to be inoculated with the disease.
From this area you look at the rest of the blade for the most advanced stage of disease as well as an estimate of the number of symptoms present (see stages). Stage
Black Sigatoka
This information is what takes the form for purposes of such calculations, scoring in the columns for each leaf (range Leaf). For each one is scored only one state, the highest and added a qualification that will be + (plus) when their density is estimated that more than 50 symptoms or-(less) when less than 50.
in banana plantations under chemical control and for this methodology is very difficult to find stages over 2 -. Only in very critical cases, probably influenced by hot spots, you will find cases of 2 +.
Symptoms usually controlled should not be taken into account during data collection, however, it is sometimes difficult to differentiate these symptoms of living. In case of uncertainty, it is preferable not to consider it and note the symptoms they are.
Although not part of the methodology or the calculations to determine the state of evolution, can also be observed and recorded, the information on the youngest leaf sick (HMJE) and the youngest leaf necrotic (HMSN), which gives a quick idea of \u200b\u200bthe condition of the plantation over Black.
From these observations, the calculations proceed as follows.
In the first evaluation, because they can not obtain the data rate of leaf emergence, as no one has the last leaf emission data (EFP), was not able to get the State Assessment data. What we have achieved this opportunity is the gross amount (SB).
The second data collection, as well as the following, you can calculate the rhythm of leaf (REF) for each plant, adding and subtracting from the current week's data (EFA-Emission Current Leaf), the data leaf emission obtained for the same plant, the previous week (EFP = Past Issue Leaf).
EFA-VET REF =
Adding the REF for each plant, and dividing this sum (S REF) by the number of days (N) that elapsed between the two observations, the data obtained Emission Rate Foliar Present (REFI) for the batch of plants under observation. REFI = SREF
N
then the information must be generated called Candela Correction (CC). This is obtained by multiplying in each plant, the value assigned to its cigarette or candle
by the number of leaves (II, III and IV) found with symptoms of the disease.
For example, if the plant A5, candela state corresponded to 4, the 3-leaf disease were observed, then CC = 4x3 = 12.
From CC data, we obtain the coefficient of Evolution (CE). This is achieved by multiplying the sum of CC for all plants by 2, which is a preset factor.
CE = S CC x 2
To finish with the necessary information related to the development of the plant (leaf development), is just get the data of Foliar Weighted Emission Rate (REFp), which is only the promise of Rate the amount of leaf present.
REFp = REF + REFI 2
The information in plant development (leaf emergence) is required to combine with the factors of clinical expressed in the plant, leading to obtain the Statement of Changes (EE) of the disease and led to a graph representing the behavior going development of the disease in the plantation.
This other process starts with the calculation of the gross amount (SB), for it is necessary to add the same amount of leaf numbering in all 10 plants tested showed the same stage (columns in the form number of damaged leaves), 5 with stage III leaves 1 +, for example.
The sum of the product of the multiplication of these data, the severity coefficients, given below for each sheet and each stage results in the gross amount (SB).
Table 2. Severity
coefficients for calculating the gross amount. Leaf Stadium
II III IV 1 to 60 40 20
1 + 80 60 40
2-100 80 60
2 + 120 100 80
3 - 140 120 100
3 + 160 140 120
As an example, in a given plantation is located in the observation plot is the stage 1 - sheet II in two plants, two sheet plants III and IV leaf of two plants.
Stage 1 + is III found in the leaf of a plant and leaf of two plants IV. Stage 2 - is in the leaf III on two floors while stage 2 + is in the leaf IV 3 floors, information that is configured and calculated in Table 3.
Table 3.
Number of Leaves Leaves Damaged Damaged coefficient
Stadiums
II III IV II III IV
1 to 2 February 2120 80 40
1 + 1 2 60 80
2 - 2 160 2 + 3 130_______
To continue shall calculate the sum of Evolution, which is obtained by subtracting the coefficient of Evolution (and as calculated above) of the gross amount.
SEV = SB - CE
This data (SEV) is multiplied by the Weighted Average Rate of leaf emergence and results in the State of Evolution (EE) of the disease for that week.
EE = SEV x REFI
This figure, taken to a graph is by allowing the trend or slope of the same between two or more weeks, to define when you make an application or fungicide treatment.
Interpretation of Results
The most important factor that can decide when to apply, not the amount of infection found in plants, but the evolution of the disease, which is reflected in the curve of a graph.
There is, in principle, a critical threshold. Only experience levels may be setting some special consideration to be defined for different sectors.
is logical that a lower level to maintain the state of development, better health will be planting and, consequently, fewer applications and fungicide treatments. This is achieved only by the applications in the most timely manner possible.
ASSESSMENT OF THE STATE OF INFECTION
You must have a clear and accurate picture of the health status of the property, to prevent severe damage to the crop and its production. For this reason we must make periodic evaluations (every week or two) on the incidence and severity of Black Sigatoka on each farm.
Below is the modified Stover methodology for the evaluation of incidence and severity of the disease, which will allow us to be properly built on the plant health of planting.
METHODOLOGY OF PROPERTY BY STOVER Gauhl
The incidence and severity assessment using the modified Stover methodology, yields fairly detailed information on the health status of the plantation.
Figure 1 shows the six degrees that includes the scale changed Gauhl Stover (1989).
The system consists of a visual estimation of leaf area affected in all the leaves around flowering plants, without having to download the sheet.
This assessment takes into account all the leaves except the leaf present fire, and leaves overwhelmed. The nearest leaf to leaf blade is considered candela No.1
FIGURE 1. Scale changed Gauhl Stover (1989).
. Counting is easy considering the spiral distribution (odd and even) from right to left from sections 1 and 2 (Figure 2), counting down. To determine the leaf area affected must visually estimated the total area covered by all the symptoms of the disease in each sheet and calculate the percentage of leaf covered by the symptoms. For this it is necessary to have a pattern that divides the sheet into percentage ratios, as shown in Figure 1.
The following is an example of the evaluation sheet (Table 1), as well as calculations to be performed to obtain the variables generated by this method.
TABLE 1. Example of calculating the number of leaves per plant (H / P).
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P
1 0 0 0 0 0 0 0 0 1 1 2 2 12 2 0 0
0 0 0 0 0 0 0 0 1 2 12
3 0 0 0 0 0 0 0 0 0 0 0 0 2 13
4 0 0 0 0 0 0 0 0 0 0 0 1 1 2 14
5 0 0 0 0 0 0 0 0 0 1 1 2 2 13 total 64
averaged 12.8
The number in each box indicates the degree of infection that has each page based on a scale of 0 a6 according to Figure 1.
To obtain the number of leaves per plant, all leaves counted and divided by the number of plants tested (Table 1). The number of leaf per plant was extracted from the last sheet is indicated (colored) in the formula assessment.
youngest diseased leaf (HMJE) is also on average younger leaves (colored) showing visible symptoms of the disease. (Table 2).
The HMJE gives an indication of disease progression. In other words, the younger the leaf with more symptoms, the greater the incidence of the disease, and arguably also the severity.
TABLE 2. Calculation of the youngest leaf sick (HMJE).
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P HMJE
1 0 0 0 0 0 0 0 0 1 1 2 2 9 2 0 0 0
0 0 0 0 0 0 0 1 2 11
3 0 0 0 0 0 0 0 0 0 0 0 0 2 13
4 0 0 0 0 0 0 0 0 0 0 0 1 1 2 12
5 0 0 0 0 0 0 0 0 0 1 1 2 2 10 total 55
average 11
Finally, to obtain the percentage of leaves infected by grade, the number of leaves in each grade, divided by the total number leaves and multiplied by 100 (Table 3).
The total percentage of infected leaves were obtained by adding the value of all grades, from first to sixth.
TABLE 3. Calculation of the percentage of infected leaves (% HJ)
NUMBER OR POSITION OF THE BLADE
Plant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H / P HMJE 0 1 2 3 4 5 6
1 0 0 0 0 0 0 0 0 1 1 2 2 8 2 2
2 0 0 0 0 0 0 0 0 0 0 1 2 10 1 1
3 0 0 0 0 0 0 0 0 0 0 0 0 2 12 0 1
4 0 0 0 0 0 0 0 0 0 0 0 1 February 1, 1911 February 1
5 0 0 0 0 0 0 0 0 0 1 1 2 2 9 2 2 Total 50 7 July
averaged 78 November 1911
EXAMPLE: Percentage of leaves with grade 1: Floor 1
: 2 leaves
Floor 2: 1 sheet
Floor 3: 0 sheets floor
4: 2 sheets
Plant 5: 2 TOTAL 7
leaves leaves with grade 1
evaluated TOTAL SHEETS: 64 SHEETS WITH GRADE
% 1 = 7 x 100 = 11.0
64 TOTAL = SUM% leaves infected leaves in each grade (from 1 to 6) = 11 + 11 + 0 + 0 + 0 + 0 = 22
However, the total percentage of infected leaves underestimates the severity of the disease and that is why the use of a weighted average of infection (PPI) has been suggested to obtain a more accurate value. Its calculation is obtained by multiplying the percentage of leaves in each grade by the corresponding value of the magnitude of modified Stover. Each result is added and the total is divided by 100.
EXAMPLE: Weighted Average of infection (PPI)
SUM (% x blades in each respective grade level)
PPI = 100 (11 x 1) + (11 x 2) +...... .. + (0 x 6) PPI = 100
PPI = 0.33
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